Embryogenesis and Plantlet Formation through Direct Cultue of Isolated Pollen of Nicotiana tabacum cv. Samsum and Nicotiana rustica cv. Rustica
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概要
- 論文の詳細を見る
Pollen embryos and plantlets of Nicotiana tabacum cv. Samsun and Nicotiana rustica cv. Rustica were obtained through direct pollen culture without prior treatment or prior culture of anthers or buds. Isolated pollen was cultured first in a medium without sucrose, then transferred into Nitsch's H medium containing 2% sucrose and 5 mM glutamine. The optimum medium for the initial culture was water and the optimum period of culture was ca. 6 days when binucleate pollen was used.
- 日本植物生理学会の論文
著者
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HARADA Hiroshi
Institute of Biological Sciences, University of Tsukuba
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Imamura Jun
Institute Of Biological Sciences The University Of Tsukuba
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Kyo Masaharu
Institute Of Biological Sciences The University Of Tsukuba
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Okabe Ekuko
Institute of Biological Sciences, The University of Tsukuba
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Okabe Ekuko
Institute Of Biological Sciences The University Of Tsukuba
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Harada Hiroshi
Institute Of Biolo Gical Sciences The University Of Tsukuba
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