Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer.
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概要
- 論文の詳細を見る
Protoplasts isolated from suspension cultured cells of orange (Citrus sinensis Osb.) were treated with a bacterial plasmid DNA carrying a chimeric gene consisting of the nopaline synthase promoter, the aminoglycoside phosphotransferase II [APH(3′)II] structural gene from the bacterial transposon Tn 5 and a terminator region from cauliflower mosaic virus DNA. Colonies capable of proliferating in a medium containing kanamycin (25μg/ml) possessed APH(3′)II activity and an intact foreign gene. The transformation frequency was recorded to be in the order of 10-6.
- 日本遺伝学会の論文
著者
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Uchimiya Hirofumi
Institute For Molecular And Cellular Biosciences University Of Tokyo
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KOBAYASHI Shozo
Akitsu Branch, Fruit Tree Research Station
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- Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer.