分離分析のための分子ふるいに関する研究(第4報) : 色素, ペプチド類のゲル濾過における電解質の影響

概要

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Effect of inorganic electrolytes on the interaction between Sephadex gels and solutes was examined in respect to gel filtration of several food dyes, oligo- and poly-peptides. Elution volume (V_e) of all the food dyes investigated and certain peptides involving lysine-vasopressin showed an increase to some appreciable extent according to lyotropic series of the anions (NCS^-<Cl^-<SO_4^<2->) in eluates. The linear relationships were obtained in the plots of the V_e values of the dyes on Sephadex G-25 and G-50 against swelling characteristics of the gels ; namely, effective diffusion coefficient of media in xerogels and ζ-potential of the gel particles in the electrolyte solutions. Because the diffusion coefficients of these dyes and peptides in these electrolyte solutions were smaller than those in deionized water solutions by porous disk method, it was suggested that the increase of the V_e values described above was mainly due to the enhancement of the aromatic sorption accompanied by a suppression of the charge effect in the presence of electrolytes. As a practical application of the effect of electrolytes on aromatic sorption, oxytocin and lysine-vasopressin were effectively separated on Sephadex G-50 column by the use of 0.1 F K_2SO_4 as an eluate, based on the difference in the location of a phenylalanyl residue in the latter peptide.
公益社団法人日本薬学会の論文
1970-10-25