Fluorescamineを用いる動物組織内残留Sulfisomezoleのけい光定量

概要

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Reaction conditions for the fluorometric determination of sulfisomezole with fluorescamine were examined, and analytical methods for sulfisomezole were established. These methods were applied to the measurements of 0.1 ppm level of sulfisomezole in animal tissue, and the content of sulfisomezole and total sulfisomezole in egg yolk, egg albumen, chicken tissues (kidney, liver, heart, gizzard, spleen, muscle, skin, and fat), and pig tissues (kidney, liver, heart, stomach, spleen, muscle, and deodenum) were determined. Sulfisomezole in these tissues was extracted with AcOEt, CH_2Cl_2,or C_2H_4Cl_2. These methods are simpler and 10 times more sensitive than the colorimetric method using Bratton-Marsall or Tsuda reagent, and are not interfered by amino acids and aliphatic amines. These methods were also used to trace the quantity of residual sulfisomezole in pig tissues after intramuscular injection or oral administration.
社団法人日本薬学会の論文
1977-05-25