多価アルデヒド類による定量法の研究(第12報)ホモフタルアルデヒドによる第一アミンのけい光定量

概要

論文の詳細を見る
Primary amines react with homophthalaldehyde in 100% or 50% acetic acid to yield 2-substituted isoquinolinium derivatives which are classified into three groups; group A (excitation (Ex.) at 340 nm, emission (Em.) at 380 nm), group B (Ex. 310 and 350 nm, Em. 400 nm), and group C (Ex. 350 nm, Em. 500nm). Best conditions for determinations of each group were examined, based on the difference in fluorescence characteristics of the reaction products and conditions. Selective and sensitive fluorometric method for determination of benzylamine, sulfanilamide, and p-anisidine with homophthalaldehyde was established. The procedure for this determination is as follows : i) To 2 ml of acetic acid solution of benzylamine, 2 ml of a 40 mg/100 ml reagent solution (homophthalaldehyde in acetic acid) is added, mixed well and heated for 40 min at 60°. The mixture is cooled and diluted to 10 ml with acetic acid, and fluorescence intensity is determined (at Ex. 343 nm, Em. 380 nm). ii) To 4 ml of 50% acetic acid solution of sulfanilamide, 4 ml of a 5 mg/100 ml reagent solution is added, mixed well, and left to stand for 30 min, fluorescence intensity of this solution is determined (at Ex. 304 nm, Em.404 nm). iii) To 4 ml of acetic acid solution of p-anisidine, 4 ml of a 15 mg/100 ml reagent solution is added, mixed well, the mixture is allowed to stand for 20 min, and its fluorescence intensity is measured (at Ex. 350 nm, Em. 478 nm).
公益社団法人日本薬学会の論文
1976-09-25

多価アルデヒド類による定量法の研究(第12報)ホモフタルアルデヒドによる第一アミンのけい光定量

スポンサーリンク

概要

著者

関連論文

スポンサーリンク