Isotachophoretic Analysis of Mercaptoundecahydrododecaborate Anion in Human Serum and Urine

概要

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An isotachophoretic assay method was developed for sodium mercaptoundecahydrododecaborate (1), Na_2^<10> B_<12>H_<11>SH, which is used for boron neutron-capture therapy. The method is simple, specific and suitable for the determination of 1 as an anion in human serum and urine. Compound 1 was separated from serum and urine constituents with 0.01 M hydrochloric acid and β-alanine (leading electrolyte, pH 3.70) and 0.01 M n-caproic acid (terminating electrolyte). The recoveries of 1 from serum and urine were about 85 and 101%, respectively. The detection limit was 7×10^<-7> mol/ml in serum and urine. The procedure could also detect the oxidation product, sodium di (thioundecahydrododecaborate), at 5×10^<-7> mol/ml or more in urine. The present method was used to determine the levels of 1 in urine samples of patients with malignant brain tumor being treated by boron neutron-capture therapy.
公益社団法人日本薬学会の論文
1985-06-25