フルオレッサミンによるアンモニアのけい光定量

概要

論文の詳細を見る
Fluorometric determination of ammonia with fluorescamine. Toshiyuki SAKANO and Tameyuki AMANO (Shionogi Research Laboratory, Shionogi and Co., Ltd., Sagisu, Fukushima-ku, Osaka-shi, Osaka) luorescence reaction of ammonia with fluorescamine (FL) in a mixed solvent of water-acetonitrile-acetone (1 : 9 : 10) was found. On the bases of this reaction, a fluorometric determination of ammonia has been developed. Two milliliters of a sample solution dissolved in 90 % acetonitrile is added to 2 ml of acetone solution of FL. The mixture is heated for 30 min at 50 °C and cooled to room temperature. The fluorescence intensity of the solution is measured at 470 nm with excitation at 380 nm. The calibration curve was found to be linear in the range of 0.01 μg / ml to 1.0 μg / ml. (Received Sept. 3, 1980)
社団法人日本分析化学会の論文
1981-02-05