3-アミノ-2(1H)-キノリンチオンによるけい光分析(第2報)p-アミノフェノールおよびアセトアミノフェンのけい光定量

概要

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A fluorometric method for the determination of p-aminophenol (AP) with 3-amino-2 (1H)-quinolinethione (AQT) was described. AP was oxidized with ceric ammonium sulfate dihydrate and allowed to react with AQT in the presence of a small amount of hydrogen peroxide in a dilute sulfuric acid solution. In an alkaline solution, the reaction product developed a yellow green fluorescence which had an excitation maximum at 394 nm and an emission maximum at 530 nm. The fluorescence intensity was linear over the range 0.5-4.5μg/ml of AP. On the other hand, acetaminophen (AAP) was hydrolyzed to AP in a dilute sulfuric acid solution, and 0.5-6.0μg/ml of AAP was determined by the same method as AP. The coefficients of variation were 1.0% (n=10) and 1.2% (n=10) for 3.0μg/ml of AP and AAP, respectively. The fluorometric method for AAP was applicable to the determination of AAP in anti-cold pharmaceutical preparations.
社団法人日本薬学会の論文
1980-03-25