α-アミノチオフェノールによるけい光分析(第5報)ニトロフラゾンのけい光定量

概要

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Fluorometric analysis of nitrofurazone with o-aminothiophenol was investigated. Nitrofurazone was hydrolyzed with hydrochloric acid to form 5-nitro-2-furaldehyde and the nitro group in this aldehyde was substituted by chlorine to form 5-chloro-2-furaldebyde, which reacted with o-aminothiophenol to form a fluorescent 2-(5-chloro-2-furyl) benzothiazole (mp 121°). On the basis of this reaction, the following method was established for determination of nitrofurazone. To 1 ml of an acetic acid solution of a sample containing 0.1-10μg of nitrofurazone, 0.5ml of conc. HCl is added, heated for 15 min, 2ml of o-aminothiophenol hydrochloride solution (0.2→100) is added, warmed for 1hr at 60°, cooled, diluted to 20ml with 90% (w/v) H_2SO_4,and the fluorescence of this solution (excitation maximum, 375nm, emission maximum, 422nm) is measured. By this method, 0.1-10μg of nitrofurazone per ml can be determined.
公益社団法人日本薬学会の論文
1974-06-25