活性酸素による生体障害に関する研究(第2報) : ヒポキサンチン-キサンチン・オキシダーゼのO_2-産生系における分離ランゲルハンス氏島からのインスリン分泌の阻害

概要

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When isolated pancreatic islets were exposed extracellularly for 5 min to oxygen radicals generating a system of hypoxanthine-xanthine oxidase (HX-X.O. system), glucose-induced insulin release from the islets was inhibited, indicating a high sensitivity of β-cell to the oxygen radicals. Addition of superoxide dismutase (SOD) together with catalase to the HX-X.O. system almost completely prevented the inhibition of the insulin release, suggesting that the islets were injured by some oxygen radicals produced by the reaction with O_2- and H_2O_2. Iron-chelator, diethylentriaminepentaacetic acid (DETAPAC) and iron-binding protein, apotransferrin also protected the inhibition of the insulin release, suggesting the possible participation of a trace iron in the cell injury caused by 0_2 and H_2O_2. Mannitol, the scavenger of hydroxyl radicals, protected the cell from oxidative damage but methylthiourea and dimethylthiourea had little effect. These results suggest that some oxygen radicals generated an extracellularly attack to the pancreatic islets and result in the inhibition of glucose-induced insulin release.
公益社団法人日本薬学会の論文
1988-02-25