Zinc Inhibition of Respiratory Burst in Zymosan-Stimulated Neutrophils : A Possible Membrane Action of Zinc
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概要
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The effect of Zn^<2+> on the respiratory burst of rat pleural neutrophils was studiesd. Serum treated zymosan (STZ)-stimulated O_2 consumption was inhibited by Zn^<2+> depending on hte Zn^<2+> concentration and time of cell incubation. Addition of Zn^<2+> after the stimulation of cells with STZ did not inhibit the O_2 consumption Zn^<2+> failed to affect the cell viability or the opsonizing process of STZ, indicating that the inhibition of O_2 consumption was not due to te direct cytotoxic action of Zn^<2+> or to the interaction of Zn^<2+> with STZ. In addition, the activity of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, an enzyme responsile for the respiratory burst of neutrophils, was not inhibited by Zn^<2+>. These results suggested that Zn^<2+> may inhibit some process of the activation of NADPH oxidase.Equimolar Zn^<2+> 8-hydroxyquinoline complex (Zn-8HQ), which is known to be unable to penetrate the cell membrane, inhibited the O_2 consumption more drastically than did Zn^<2+> alone. 8-Hydroxyquinoline alone did not cause significant inhibition of O_2 consumption, indicating that the inhibitory effect of Zn^<2+> is potentiated by complexing with 8-hydrozyquinoline. The inhibition of O_2 consumption Zn^<2+> was almost completely restored by washing and reincubating the Zn^<2+>-treated cells in Zn^<2+>-free medium. On the other hand, in the cells treated with Zn-8HQ, the same treatment of cell washing and reincubation only partially restored the O_2 consumption. These results suggested that Zn^<2+> may be acting on the cell membrane of neutrophils.
- 社団法人日本薬学会の論文
- 1988-03-25
著者
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小木曽 健人
Hokkaido Institute Of Pharmaceutical Sciences
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八柳 潤
Hokkaido Institute Of Pharmaceutical Sciences
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