Inhibition of Superoxide Generation and of Increase in Intracellular Ca^<2+> Concentration by Zinc in Rat Neutrophils Stimulated with Zymosan

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The effect of Zn^<2+> on the O^-_2 generation and change in intracellular Ca^<2+> concentration ([Ca^<2+>]_i) of rat peritoneal neutrophils was studied. Zymosan (serum-treated zymosan (STZ))-induced O^-_2 generation was inhibited by Zn^<2+> at concentrations as low as 10μM. A large amount of the inhibition was observed in the absence of extraceullular Ca^<2+> but the inhbition could not be restored by increasing the extracellular Ca^<2+> concentration, indicating that Zn^<2+> does not necessarily inhibit the O^-_2 generation competitively with extracellular Ca^<2+>. In the absence of extracellular Ca^<2+>, Zn^<2+> inhibited STZ-induced transient increase in [Ca^<2+>]_i in the concentration range that evoked a marked inhibition in the O^-_2 generation. On the other hand, Zn^<2+> did not inhibit significantly STZ-induced uptake of ^<45>Ca^<2+> from extracellular medium by the cells.From these results, it is suggested that Zn^<2+> inhibits STZ-induced release of Ca^<2+> from intracellular strage sites, resulting in the suppression of the activation mechanism of neutrophils.
社団法人日本薬学会の論文
1990-06-25