Roke of Alloxan Radical in Generation of Hydroxyl Radical by Reacion of Alloxan with Glutathione in the Presence of Fe^<3+>-Ethylenediaminetetraacetic Acid

概要

論文の詳細を見る
The degradation of deoxyribose, an indicator of hydroxyl radiacal(HO・)generation, was observed in the reaction of alloxan with glutathione (GSH) in the presence of Fe^<3+>-ethylenediaminetetraacetic acid (EDTA). Catalase and HO・scavengers strongly inhibited this degradation, but superoxide dismutase (SOD) did not do so effectively, suggesting that the HO・ was generated via the Fenton reaction but not the Haber-Weiss reaction. The reduction of Fe^<3+>-EDTA was rapid in the reaction of alloxan with GSH. The generation of alloxan radical in the reaction system was diminished by the addition of Fe^<3+>-EDTA, indicating that Fe^<3+>-EDTA was directly reduced by the alloxan radical . However, only a large amount of SOD inhibited both reduction of Fe^<3+>-EDTA and generation of alloxan radical, suggesting that superoxide radical(O^-_2)may participate indirectly in both reactions. The generation of O^-_2 and H_2O_2 in the reaction was confirmed by the formation of compound III from lactoperoxidase and by partial regeneration of consumed oxygen upon addition of catalase, respectively, suggesting that O^-_2 may be a source of H_2O_2. From these results, we concluded that HO・ was generated by alloxan radical-dependent Fenton reaction.
公益社団法人日本薬学会の論文
1990-04-25