Enhanced Lipid Peroxidation of Erythrocyte Membranes and Phosphatidylcholine Liposomes Induced by a Xanthine Oxidase System in the Presence of Catalase
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概要
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When rat erythrocyte membranes or phosphatidylcholine (PC) liposomes were incubated in a xanthine oxidase system, lipid peroxidation was stimulated by the addition of catalase. Addition of a large amount of xanthine oxidase caused no significant increase in the lipid peroxidation of PC liposomes unless catalase was present in the reaction system. Enhanced peroxidations of both membranes and liposomes observed in the presence of catalase were strongly inhibited by superoxide dismutase (SOD), suggesting that O^-_2 is an essential species in these reactions. Several iron-chelators strongly inhibited the lipid peroxidations, suggesting an involvement of iron in the peroxidation reaction. Unless catalase was present, the rate of liposome peroxidation was stimulated only slightly by addition of Fe^<3+>, but in the presence of catalase, the addition of Fe^<3+> markedly accelerated the peroxidation reaction, which was inhibited by SOD or desferrioxamine. Furthermore, the addition of Fe^<3+> to the xanthine oxidase system in the presence of catalase caused a significant decrease in the rate of cytochrome c reduction, suggesting that Fe^<3+> may be reduced to Fe^<2+> by O^-_2. The peroxidations enhanced by catalase may be induced by supply of sufficient O^-_2 and Fe^<2+> for lipid peroxidation. Histidine and 1,4-diazabicyclo-(2,2,2)-octane, quenchers of ^1O_2,inhibited the peroxidation of membranes and liposomes. Mannitol and benzoate, scavengers of OH^・, showed no significant effect on the peroxidation of membranes or liposomes. These results indicate possible participation of ^1O_2 species in the O^-_2-dependent lipid peroxidation induced by the xanthine oxidase system in the presence of catalase.
- 公益社団法人日本薬学会の論文
- 1984-08-25
著者
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桜井 光一
北海道薬科大学
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小木曽 健人
Hokkaido Institute Of Pharmaceutical Sciences
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桜井 光一
Hokkaido Institute of Pharmaceutical Sciences
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三浦 俊明
北海道薬科大学
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桜井 光一
北海道薬科大学生化学研究室
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Ogiso T
Hokkaido Institute Of Pharmaceutical Sciences
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