Alterations of Specific and Nonspecific Binding of Monoclonal Antibody by Introduction of Acidic and Hydrophobic Groups

概要

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AB-6 monoclonal antibody (mAb) against bovine serum albumin (BSA) was coupled with β-alanine or n-propylamine using a heterobifunctional cross-linking reagent, N-(ε-maleimidocaproyloxy) succinimide (EMCS) and a linker, poly-L-cysteine to introduce acidic or hydrophobic groups into the mAb. The mAb molecules coupled with β-alanine at lower than 1 : 16 molar ratios and those coupled with n-propylamine at 1 : 14 retained the original reactivity to BSA but modification with higher amounts of β-alanine or n-propylamine decreased the reactivity. In contrast, nonspecific bindings of AB-6 mAb to a plastic culture plate and tumor (MDA-MB-453) cells were decreased by modification with β-alanine and increased by n-propylamine. These results suggested that introduction of acidic groups, and hydrophobic groups to antibody leads to fluctuations in the non-specific binding of antibody.
公益社団法人日本薬学会の論文
1994-06-15