Dual Effects of Cycloheximide on U937 Apoptosis Induced by Its Combination with VP-16(Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
In this study, cycloheximide (CHX) and VP-16 alone and in combination (C&V) have been used to strongly trigger apoptosis in U937 cells. The presence of CHX markedly prevented VP-16-induced apoptosis, suggesting that in this process de novo protein synthesis is required. But interestingly, C&V had shown more similarities with CHX but not VP-16 alone, including the effects on cell cycle distribution and induction of apoptosis, which occurred more quickly and was steadily enhanced by increasing concentrations of CHX or by N-α-tosyl-L-lysylchloromethyl ketone (TLCK), a serine protease inhibitor. These results indicate that CHX, not VP-16, is indeed the dominant inducer of U937 apoptosis, when they are coadministered. In particular, VP-16 even promoted CHX-induced apoptosis, but did not alter its selection of cell types. In T-cells resistant to CHX (Molt-4), we have detected no apoptotic response to their combination. These findings may well explain why the inhibitory effects of CHX on apoptosis induced by the same stimuli are usually different according to the cell type used, and also suggest that CHX may have the potential to lower side effects and drug resistance of cancer therapy.
- 公益社団法人日本薬学会の論文
- 2004-07-01
著者
-
Wu Rong-cong
Institute Of Biomedicine Tsinghua University:department Of Biological Sciences And Biotechnology Tsi
-
Liu Ming-jie
Institute Of Biomedicine Tsinghua University:department Of Biological Sciences And Biotechnology Tsi
-
Chen Di-fei
Institute Of Biomedicine Tsinghua University:department Of Biological Sciences And Biotechnology Tsi
-
Wang Zhao
Institute Of Biomedicine Tsinghua University:department Of Biological Sciences And Biotechnology Tsi
-
Wang Zhao
Protein Sci. Key Lab. Of The Ministry Of Education School Of Medicine Tsinghua Univ.
関連論文
- Effects of Two Saponins Extracted from the Polygonatum Zanlanscianense Pamp on the Human Leukemia (HL-60) Cells
- Gastrointestinal Absorption Characteristics of Glycyrrhizin from Glycyrrhiza Extract
- Mechanism of Gastrointestinal Absorption of Glycyrrhizin in Rats
- Overexpression of QM Induces Cell Differentiation and Mineralization in MC3T3-E1(Molecular and Cell Biology)
- Cloning and Characterization of Testis-Specific Spermatogenesis Associated Gene Homologous to Human SPATA4 in Rat(Biochemistry/Molecular Biology)
- Alterations of Specific and Nonspecific Binding of Monoclonal Antibody by Introduction of Acidic and Hydrophobic Groups
- Cytoplasm Prepared from All-trans Retinoic Acid (ATRA)-Treated Human Hematopoietic Progenitor Cells Induces Differentiation of Both ATRA Sensitive and ATRA Resistant Leukemia Cells (Toxicology)
- The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines(Pharmacology)
- Dual Effects of Cycloheximide on U937 Apoptosis Induced by Its Combination with VP-16(Pharmacology)
- Molecular Cloning and Expression of a Pearl Oyster (Pinctada fucata) Homologue of Mammalian Putative Tumor Suppressor QM
- Apoptosis Induced by Dioscin in Hela Cells
- Pinctada fucata Mantle Gene 5 (PFMG5) from Pearl Oyster Mantle Inhibits Osteoblast Differentiation
- Matrix Protein Biglycan Induces Osteoblast Differentiation through Extracellular Signal-Regulated Kinase and Smad Pathways