Overexpression of QM Induces Cell Differentiation and Mineralization in MC3T3-E1(Molecular and Cell Biology)
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概要
- 論文の詳細を見る
It has been reported that QM was highly expressed by cells isolated from epiphyseal cartilage as opposed to proliferative chondrocytes. In vitro investigation of the expression of QM revealed higher QM expression in non-mineralizing osteoblast and pericyte cultures as compared with mineralizing cultures. These evidences suggest that QM may play an essential role in cell differentiation before mineralization. However, our research results showed that QM overexpression in MC3T3-E1 enhanced cell differentiation and mineralization. In this study, alkaline phosphatase (ALP) activity and nodule mineralization were increased in MC3T3-E1 from QM overexpression cultures relative to normal expression QM cultures. RT-PCR revealed upregulation of the marker genes type I collagen, ALP, osteocalcin, osterix and BMP-2 and a slight decrease of a negative regulator osteopontin. These results suggest that the increasing of QM expression could stimulate osteoblast differentiation and mineralization in MC3T3-E1.
- 公益社団法人日本薬学会の論文
- 2005-08-01
著者
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Wang Z
Department Of Biological Sciences And Biotechnology Tsinghua University
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王 〓
Faculty Of Pharmaceutical Sciences Okayama University
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Wang Zhao
Department Of Biological Sciences And Biotechnology Tsinghua University
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Liu Shang
Department Of Biological Sciences And Biotechnology Tsinghua University:institute Of Biomedicine Tsi
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Zhao Ying
Department Of Cardiology Anzhen Hospital Capital Medical University
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GUAN Hong
Department of Biological Sciences and Biotechnology, Tsinghua University
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WU Rong
Department of Biological Sciences and Biotechnology, Tsinghua University
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LIU Shang-Feng
Department of Biological Sciences and Biotechnology, Tsinghua University
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Wang Z
Faculty Of Pharmaceutical Sciences Okayama University
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Guan Hong
Department Of Biological Sciences And Biotechnology Tsinghua University
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Zhao Yuying
Department Of Natural Medicines And The State Key Laboratory Of Natural And Biomimetic Drugs School
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Wang Zhao
Protein Sci. Key Lab. Of The Ministry Of Education School Of Medicine Tsinghua Univ.
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Wu Rong
Department Of Biological Sciences And Biotechnology Tsinghua University
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Zhao Ying
Department of Biological Sciences and Biotechnology, Tsinghua University
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WANG Zhao
Department of Anesthesiology, Northwestern University Medical School
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