Assay of Cell Surface-Bound Immunoliposomes Using Monoclonal Antibody Reactive with a Crosslinking Reagent

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概要

• 論文の詳細を見る

• A monoclonal antibody (mAb) reactive with a crosslinking reagent, N-(m-maleimidobenzoyl)dipalmitoylphos-phatidylethanolamine (mMBPE), in liposomes was produced from a hybridoma clone established by a fusion between P3X63Ag8.653 mouse myeloma cells and spleen cells from a BALB/c mouse hyperimmunized with the antibody-coated liposomes containing mMBPE. Using this mAb (termed AL-6), the quantity of immunoliposomes bound on target tumor cells was assessed by flow cytofluorometry. The results obtained using fluorescein isothiocyanate-coupled AL-6 allowed the enumeration not only of the immunoliposomes bound on all tumor cells but also those on individual target tumor cells. The relevance of this assay method was confirmed by a comparison with another assay method of cell-bound liposomes using immunoliposomes containing carboxyfluorescein in the vesicles.

• 社団法人日本薬学会の論文
• 1992-07-25

著者

• 鈴木 真也

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Tohoku University

• 橋本 嘉幸

  佐々木研

• 橋本 嘉幸

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Tohoku University:(present Addre

• 益子 高

  東北大・薬・遺伝子薬学

• Masuko Takashi

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Tohoku University

• Suzuki S

  Faculty Of Pharmacy Meiji University

• 益子 高

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tohoku University

• 高梨 一也

  Department of Hygienic Chemistry, Pharmaceutical Institute, Tohoku University

• 高塩 一俊

  Department of Hygienic Chemistry, Pharmaceutical Institute, Tohoku University

• 高梨 一也

  Department Of Hygienic Chemistry Pharmaceutical Institute Tohoku University

• 高塩 一俊

  Department Of Hygienic Chemistry Pharmaceutical Institute Tohoku University

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