Effect of α-Linolenic Acid on the Metabolism of ω-3 and ω-6 Polyunsaturated Fatty Acids and Histamine Release in RBL-2H3 Cells

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We examined the effect of α-linolenic acid (18 : 3 (n-3)) pretreatment on the metabolism of ω-3 and ω-6 polyunsaturated fatty acids and histamine content and release of RBL-2H3 cells. RBL-2H3 cells grew without reduction in number when incubated with subculture media for 3 d and then placed again in serum-free medium with bovine serum albumin (BSA) and epidermal growth factor (EGF). Cholesterol pullulan (10 μg/ml) emulsified α-linolenic acid (20 μg/ml) was recommended as an additional form serum free medium. We determined the fatty acid composition in all neutral lipids, free fatty acids and all phospholipids in α-linolenic acid-treated cells. In all cases the concentration of α-linolenic acid and docosahexenoic acid (DHA, 22 : 6 (n-3)) was increased, while linolenic acid (18 : 2 (n-6)) was slightly and arachidonic acid (20 : 4 (n-6)) was markedly decreased. Content of histamine in α-linolenic acid-treated cells was remarkably lower than that of untreated cells. Accordingly, net histamine release stimulated by antigen or A23187 was also markedly decreased in the α-linolenic acid-treated cells, as was the percent histamine release stimulated by antigen. Results from our in vitro experiment suggest that the anti-allergic effect of α-linolenic acid may be caused either by the decrease in histamine content or by inhibition of the release of chemical mediator resulting from changes in the fatty acid composition.
公益社団法人日本薬学会の論文
1994-10-15

Effect of α-Linolenic Acid on the Metabolism of ω-3 and ω-6 Polyunsaturated Fatty Acids and Histamine Release in RBL-2H3 Cells

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