Studies on the Biosynthesis of Corrinoids and Porphyrinoids. X. Biosynthetic Studies of Bacteriochlorophyll-a in Rhodopseudomonas spheroides : Incorporation of ^2H-, ^<13>C- and ^<15>N-Labeled Substrates and Origin of the Hydrogen Atoms

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The biosynthetic pathway of bacteriochlorophyll-a in Rhodopseudomonas spheroides was investigated by administration of [2-^<13>C]glycine, L-[methyl-^<13>C]methionine, L-[1-^<13>C]glutamic acid and ^<13>C- or ^<15>N-labeled δ-aminolevulinic acid (ALA). The origin of the hydrogen atoms was studied by following the incorporation into bacteriochlorophyll of ^<13>C-labeled ALA in a medium containing 50% D_2O and that of ^2H-, ^<13>C-labeled ALA. The labeled bacteriochlorophylls were converted into more stable methyl bacteriopheophorbides, which were subjected to ^<13>C-NMR analyses to obtain information about the biosynthetic origin of the hydrogen atoms. The use of ^<13>C-enriched bacteriochlorophylls and methyl bacteriopheophorbides allowed us to make ^<13>C-NMR signal assignments for all carbons of the porphyrin nucleus.
社団法人日本薬学会の論文
1995-08-15