89(P2-19) クロロフィル・バクテリオクロロフィルの生合成(ポスター発表の部)

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概要

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• We have synthesized ^<13>C-labeled precursors such as glycine, methionine and glutamic acid for the biosynthesis of porphyrinoid. We also established the regioselective synthesis of ^<13>C-labeled ALA (δ-aminolevulinic acid) in the same method from ^<13>C-labeled sodium acetate, and utilize these in the study of biosynthesis of chlorophyll and bacteriochlorophyll. L-[1-^<13>C]glutamic acid-incorporated chlorophyll showed ^<13>C-enriched peaks at sp^2 region. This result shows ring carbons are derived from glutamic acid by Beale route in E. gracilis. Incorporation of [2-^<13>C]glycine to E. gracilis gave chlorophyll labeled at 10b methyl ester (52.4 ppm). In the same way, L-[^<13>CH_3] methionine was incorporated and showed enriched peak at 52.4 ppm. It indicates glycine was metabolized into methionine and was incorporated into one side chain of chlorophyll in E. gracilis. [2-^<13>C]glycine-incorporated bacteriochlorophyll showed ^<13>C-enriched peaks at sp^2 region. Also the feeding experiment of [5-^<13>C]ALA into bacteriochlorophyll showed the same result. It indicates ALA was derived from glycine by Shemin route in R. spheroides. Furthermore feeding experiment of ^<13>C-labeled ALA in 50% D_2O medium and that of ^<13>C, ^2H-doubly labeled ALA into bacteriochlorophyll showed α,β isotope-shifted peaks at ring carbons. It showes ring protons at C5a, C3, C4, C8 position of bacteriochlorophyll are derived from water in R. spheroides.

• 天然有機化合物討論会の論文
• 1989-09-17

著者

• 車谷 勝行

  Department of Medicinal Chemistry, Meiji College of Pharmacy

• 車谷 勝行

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

• 梶原 正宏

  明治薬大

• 岡崎 健男

  Department of Medicinal Chemistry, Meiji College of Pharmacy

• 岡崎 健男

  明治薬大

• 寒河江 葉子

  Department of medicinal Chemistry, Meiji College of Pharmacy

• 寒河江 葉子

  明治薬大

• 車谷 勝行

  明治薬大

• 岡崎 健男

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

• 寒河江 葉子

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

• Okazaki Takeo

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

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