19 コリン・ポルフィリン類の生合成(ポスター発表の部)

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概要

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• We have established ragioselective synthesis of [^<13>CJALA labeled at 1,2,3,4, and 5 positions (as the important precursors for the biosynthesis of corrinoid and porphyrinoid) by a simple, and complementar method, staring from ^<13>C sodium acetate. We also complete the whole assignment of complicated ^1H-NMR signals of Vitamin B_<12'> by the incorporation of labeled precursors and the 2D-NMR measurements. We isolated and partly purified the enzymes which transform ALA to PBG and Uro'gen I and could observe their time-pass changing by NMR. We have been interested in the origin of hetero atom of vitamin B_<12>. We revealed the origin of proton of corrin ring. The 3, 87, 13, 18, and 19 positioned protons are derived from water in the culture. We also made clear that the amide oxygens of vitamin B_<12> are derived from ALA without exchanging, except that of acetyl group of A ring, which result supports the hypothesis of lactone-intermediate suggested by Eschenmoser. We studied the origin of nitrogen, and could observe the ^<15>N-NMR signals of the nitrogens of pyrrole, nucleotide, and cyano group of vitamin B_<12>. We found that ALA was biosynthesized from glutamate, and glycine is metabolized to methionine, which was transformed into the 10b methyl ester of chlorophyll-a in Euglena gracilis, from a number of feeding experiments. We also confirmed ALA was incorporated into bacteriochlorophyll-a in Rhodo. spheroides.

• 天然有機化合物討論会の論文
• 1988-09-26

著者

• 車谷 勝行

  Department of Medicinal Chemistry, Meiji College of Pharmacy

• 車谷 勝行

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

• 梶原 正宏

  明治薬大

• 岡崎 健男

  Department of Medicinal Chemistry, Meiji College of Pharmacy

• 岡崎 健男

  明治薬大

• 車谷 勝行

  明治薬大

• 岡崎 健男

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

• Okazaki Takeo

  Department Of Medicinal Chemistry Meiji College Of Pharmacy

関連論文

• Synthesis of [^C]Phenacetin and Its Application ot the Breath Test for the Diagnosis of Liver Disease
• Stereochemical Studies on the Formation of Melanin by Monophenol Monooxygenase
• 97(P16) タキサジエンの合成研究(ポスター発表の部)
• P-54 コリン、ポルフィリン類の生合成(ポスター発表の部)
• Studies on the Biosynthesis of Corrinoids and Porphyrinoids. X. Biosynthetic Studies of Bacteriochlorophyll-a in Rhodopseudomonas spheroides : Incorporation of ^2H-, ^C- and ^N-Labeled Substrates and Origin of the Hydrogen Atoms
• 66 コリン・ポルフィリン類の生合成(ポスター発表の部)
• Studies of the Biosynthesis of Corrinoids and Porphyrinoids. IV. Biosynthesis of Chlorophyll in Euglena gracilis
• Studies on the Biosynthesis of Corrinoids and Porphyrinoids. III. The Origin of Amide Nitrogen of Vitamin B_
• Direct Observation of Enzymatic Transformation of δ-Aminolevulinic Acid to Porphobilinogen and Uroporphyrinogen in a Cell-Free System by Carbon-13 Nuclear Magnetic Resonance Spectroscopy
• ビタミンB_生合成機構解明の現状
• Studies on the Biosynthesis of Corrinoids and Porphyrinoids.II. : The Origin of Nitrogen of Vitamin B_
• 89(P2-19) クロロフィル・バクテリオクロロフィルの生合成(ポスター発表の部)
• Studies on the Biosynthesis of Corrinoids and Porphyrinoids. I : The Labeling of Oxygen of Vitamin B_
• 2-I-45 ビタミンB_の生合成 : ヘテロ原子の由来について(第41回大会一般研究発表)
• Proton Nuclear Magnetic Resonance (^1H-NMR) Signal Assignment of Vitamin B_ Based on Normal Two-Dimensional NMR and Feeding Experiments
• 19 コリン・ポルフィリン類の生合成(ポスター発表の部)
• Studies on ^C-Phenacetin Metabolism. II. A Combination of Breath Test and Urine Test of in Vivo Metabolites in the Diagnosis of Liver Disease

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