Studies of the Biosynthesis of Corrinoids and Porphyrinoids. IV. Biosynthesis of Chlorophyll in Euglena gracilis

概要

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The biosynthetic pathway of chlorophylla-a formation in the photosynthetic phytoflagellate Englena gracilis was investigated by administration of ^2H- and ^<13>C-labeled precursors. L-[1-^<13>C]Glutamate was incorporated into eight carbon atoms in the chlorophyll macrocycle via δ-aminolevulinic acid formed through the C-5 pathway. A low incorporation of D-[1-^<13>C]glutamate was also observed. [2-^<13>C]Glycine was preferentially incorporated into the methyl ester carbon of chlorophyll via the one-carbon metabolic pathway, but not via the Shemin pathway. Feeding experiments with ^2H-, and ^2H, ^<13>C-labeled glycine yielded labeled chlorophylls, which were subjected to nuclear magnetic resonance and mass spectra analyses to obtain detailed information about the biosynthetic orgin of the hydrogen atoms.
社団法人日本薬学会の論文
1990-12-25