Proton Nuclear Magnetic Resonance (^1H-NMR) Signal Assignment of Vitamin B_<12> Based on Normal Two-Dimensional NMR and Feeding Experiments
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概要
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Proton nuclear magnetic resonance (^1H-NMR) signal assignment of cyanocobalamin (vitamin B_<12>) was achieved by the normal two-dimensional NMR method. ^1H-^1H correlation spectroscopy (COSY) of vitamin B_<12> showed correlation networks of dimethyl benzimidazole, ribose, isopropanolamine, and corrin ring proton peaks. ^1H-^1H nuclear Overhauser effect (NOE) spectroscopy (NOESY) allowed assignment of the benzimidazole protons. For assignment of methyl and methylene protons of the corrin ring, ^1H-^<13>C COSY measurements were made on ^<13>C-enriched vitamin B_<12>, isolated after feeding experiments with [^<13>CH_3]methionine, [2-^<13>C]5-aminolevulinic acid (ALA), and [3-^<13>C]ALA.
- 社団法人日本薬学会の論文
- 1989-01-25
著者
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車谷 勝行
Department of Medicinal Chemistry, Meiji College of Pharmacy
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梶原 正宏
Department of Medicinal Chemistry, Meiji College of Pharmacy
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車谷 勝行
Department Of Medicinal Chemistry Meiji College Of Pharmacy
-
梶原 正宏
Department Of Medicinal Chemistry Meiji College Of Pharmacy
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