Detection of 8-Hydroxy-2'-deoxyguanosine in Deoxyribonucleic Acid by the ^<32>P-Postlabeling Method
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概要
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Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the ^<32>P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxy-ribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 10^4 DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.
- 社団法人日本薬学会の論文
- 1991-07-25
著者
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幸田 光復
Faculty of Pharmaceutical Sciences, Nagoya City University
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幸田 光復
Faculty Of Pharmaceutical Sciences Nagoya City University
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LU Lee-Jane
Division of Environmental Toxicology
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田坂 文代
Faculty of Pharmaceutical Sciences, Nagoya City University
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HOKANSON James
Division of Epidemiology and Biostatistics
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田坂 文代
Faculty Of Pharmaceutical Sciences Nagoya City University
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Lu Lee-jane
Division Of Environmental Toxicology:department Of Preventive Medicine And Community Health The Univ
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Hokanson James
Division Of Epidemiology And Biostatistics:department Of Preventive Medicine And Community Health Th
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