Hepatic Calcium-Binding Protein Regucalcin Decreases Ca^<2+>/Calmodulin-Dependent Protein Kinase Activity in Rat Liver Cytosol

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The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on cytosolic Ca^<2+>/calmodulin-dependent protein kinase activity was investigated. The increase in cytosolic Ca^<2+>/calmodulin-dependent protein kinase activity with passage of incubation time was clearly prevented by the presence of regucalcin (1.0 μM). An appreciable effect of regucalcin was seen at 0.5 μM. The cytosolic Ca^<2+>/calmodulin-dependent protein kinase activity was fairly increased by increasing concentrations of added Ca^<2+> (0.25-1.0 mM). This increase was clearly blocked by the presence of regucalcin (1.0 μM). The inhibitory effect of regucalcin on the protein kinase activity was also seen with varying concentrations of calmodulin (2.5-15 μg). In the presence of regucalcin (1.0 μM), trifluoperazin (50 μM), an antagonist of calmodulin, significantly decreased the cytosolic Ca^<2+>/calmodulin-dependent protein kinase activity. These results suggest that regucalcin can regulate the Ca^<2+>-calmodulin effect in liver cytosol.
公益社団法人日本薬学会の論文
1990-08-25