Comparison of the Effect of β-Alanyl-L-histidinato Zinc and Its Zinc-Chelating Ligand on Bone Metabolism in Tissue Culture

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The present investigation was undertaken to compare the effects of β-alanyl-L-histidinato zinc (AHZ) and its zinc-chelating ligands on bone metabolism in tissue culture. Calvaria were removed from 3-week-old male rats and cultured for up to 72h in Dulbecco's modified eagle medium containing zinc sulfate, AHZ, di (N-acetyl-β-alanyl-L-histidinato) zinc (AAHZ), and di (histidino) zinc (HZ). The bone calcium content and alkaline phosphatase activity were significantly increased in the presence of AHZ or AAHZ (10^<-8>-10^<-5> M). Those increases were seen at 10^<-7> to 10^<-5> M zinc sulfate and HZ. The bone deoxyribonucleic acid (DNA) content was significantly increased by AHZ or AAHZ with 10^<-7> to 10^<-5> M, while 10^<-7> M zinc sulfate and HZ had no effect. Thus, AHZ and AAHZ had more potent effect than that of zinc sulfate and HZ. The effect of AHZ, AAHZ and HZ (10^<-5> M) increasing bone alkaline phosphatase activity was abolished by the presence of 10^<-4> M dipicolinate, a chelator of zinc. Moreover, the effect of these zinc compounds on bone metabolic indices was not seen in the presence of 10^<-6> M cycloheximide. The present results suggest that the effect of AHZ and AAHZ on bone metabolism is more potent than that of zinc sulfate and HZ.
公益社団法人日本薬学会の論文
1994-04-15