Effects of Ca^<2+>, Zn^<2+> and Cd<2+> on Uridine Diphosphate-Glucuronyltransferase and β-Glucuronidase Activities in Rat Liver Microsomes
スポンサーリンク
概要
- 論文の詳細を見る
The effect of various metals on uridine diphosphate (UDP)-glucuronyltransferase and β-glucuronidase activities in rat liver microsomes was investigated. The presence of Mn^<2+>, Cd^<2+>, Zn^<2+>, V^<5+>, Ni^<2+>, Co^<2+>, Cu^+ or Ca^<2+> (20μM) in the enzyme reaction mixture did not a significant alteration of UDP-glucuronyltransferase activity in hepatic microsomes. Of these metals, Zn^<2+> and Cd^<2+> (20μM) caused a remarkable increase in hepatic microsomal β-glucuronidase activity. Appreciable effects of Zn^<2+> and Cd^<2+> on β-glucuronidase activity were seen at 5.0 μM, and the effects were saturated at 50 μM. Ca^<2+> (5.0-50 μM) and/or the Ca^<2+>-binding protein regucalcin (2.0μM) did not have an appreciable effect on UDP-glucuronyltransferase and β-glucuronidase activities in hepatic microsomes. Thus, Zn^<2+> and Cd^<2+> uniquely increased β-glucuronidase activity. The Zn^<2+>-and Cd^<2+>-induced increase in β-glucuronidase activity was completely reversed by the presence of an SH group-protecting reagent (dithiothreitl). The response of the microsomal enzyme to Zn^<2+> and Cd^<2+> (20 μM) was no longer seen after treatment with 0.2% Triton X-100 [polyoxyethylene(10) octylphenyl ether], indicating that the stimulation by these metals is dependent on membrane association. The present study suggests that, of various metals tested, Zn^<2+> and Cd^<2+> can uniquely increase hepatic microsomal β-glucuronidase actibity, and that their effect is based on binding to membranous SH groups, beside the enzyme protein.
- 公益社団法人日本薬学会の論文
- 1990-01-25
著者
-
祐田 泰延
Department of Environmental Biochemistry and Toxicology, University of Shizuoka School of Pharmaceut
-
森 聖一
Department Of Environmental Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka
-
山口 正義
Department of Environmental Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
-
山口 正義
Department Of Environmental Biochemistry And Toxicology School Of Pharmaceutical Sciences University
-
祐田 泰延
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
-
Suketa Yasunobu
Department Of Environmental Biochemistry And Toxicology University Of Shizuoka School Of Pharmaceuti
関連論文
- Changes in IgA and Metals in Serum and Urine of Human Volume Hypertension
- Isolation and Biosynthesis of L-Homomethionine (L-5-Methylthionorvaline)
- New Sulfur containing Amino Acids in Cabbage : Isolation and Identification of L-Homomethionine (L-5-Methylthionorvaline)
- Effect of Fluoride on the Activities of the Na^+/Glucose Cotransporter and Na^+/K^+-ATPase in Brush Border and Basolateral Membranes of Rat Kidney (in Vitro and in Vivo)
- Physiological Significance of Calcium Excretion into the Bile of Rats
- Dietary and Thyroparathyroidal Regulation of Calcium Excretion into the Bile of Rats
- スズ (環境汚染物質と毒性無機物質)
- A Role of Protein Kinase C in the Alteration of Renal Glucose-6-phosphatase Activity Caused by Fluoride
- Immunohistochemical Demonstration of Calcium-Binding Protein Regucalcin in the Tissues of Rats : The protein Localizes in Liver and Brain
- Calcium-Binding Protein Regucalcin Stimulates the Uptake of Ca^ by Rat Liver Mitochondria
- Regucalcin-Induced Ca^ Release from Rat Liver Microsomes : The Effect Is Inhibited by Heparin
- Hepatic Calcium-Binding Protein Regucalcin Decreases Ca^/Calmodulin-Dependent Protein Kinase Activity in Rat Liver Cytosol
- Effects of Ca^, Zn^ and Cd on Uridine Diphosphate-Glucuronyltransferase and β-Glucuronidase Activities in Rat Liver Microsomes
- Effect of the Calcium-Binding Protein Regucalcin on the Ca^ Transport System in Rat Liver Microsomes : The Protein Stimulates Ca^ Release
- Activation of Hepatic Microsomal Ca^-Adenosine Triphosphatase by Calcium-Binding Protein Regucalcin
- Effects of Ca^ and V^ on Glucose-6-phosphatase Activity in Rat Liver Microsomes : The Ca^ Effect Is Reversed by Regucalcin
- Calcium-Binding Protein Regucalcin Is an Activator of (Ca^-Mg^)-Adenosine Triphosphatase in the Plasma Membranes of Rat Liver
- Effects of Ca and Zn on 5'-Nucleotidase Activity in Rat Liver Plasma Membranes : Hepatic Calcium-Binding Protein (Regucalcin) Reverses the Ca Effect
- Alteration of Glucose Consumption and Adenosine Triphosphate Content in Bone Tissue of Rats with Different Ages : : The Stimulatory Effect of Zinc
- Regulatory Effect of Calcium-Binding protein Isolated from Rat Liver Cytosol on Activation of Fructose 1,6-Diphosphatase by Ca^-Calmodulin
- Synergistic Enhancement of Nitrite on Lysophospholipid-Mediated Cytolysis
- Effect of Nitrite on Cell Growth and Antibody Production in Mouse Splenic B Cells Stimulated with Lipopolysaccharide and B Cell Hybridomas
- Physicochemical Properties of Calcium-Binding Protein Isolated from Rat Liver Cytosol : Ca-Induced Conformational Changes
- Calcitonin Increases Succinate Dehydrogenase Activity Dependently on Calcium in the Hepatic Mitochondria of Rats(Biological,Chemical)
- Calcitonin Stimulates Adenosine-5'-triphosphate Synthesis Calcium-Dependently in the Hepatic Mitochondria of Rats(Biological,Chemical)
- Effect of Calcium-Binding Protein on the Activation of Phosphorylase a in Rat Hepatic Participate Glycogen by Ca^(Biological)
- Calcium-Binding Protein Isolated from Rat Liver Cytosol Reverses Activation of Pyruvate Kinase by Ca^(Biological)
- Mitochondrial Uptake of ^Ca^ Bound to Calcium-Binding Protein Isolated from Rat Liver Cytosol
- 金属による生体内カルシウム代謝異常の発現
- 重金属毒性解析の生化学的アプローチ(パネルディスカッション)(第10回環境汚染物質とそのトキシコロジーシンポジウム)
- 無機スズの標的臓器と作用発現
- カルチトニンによる生体内カルシウム調節の機構
- Movement of Subcellular Calcium in the Liver of Bile Duct-ligated Rats
- Zinc Accumulation and Succinate Dehydrogenase Activation in Hepatic Mitochondria of Rats orally Administered Zinc Sulfate
- Zinc Metabolism in the Liver of Rats orally administered Zinc Sulfate
- Calcitonin increases Serum Glucose Concentration independently of Insulin Secretion in Rats
- Properties of Calcium-Binding Protein isolated from the Soluble Fraction of Normal Rat Liver