Calcitonin Stimulates Adenosine-5'-triphosphate Synthesis Calcium-Dependently in the Hepatic Mitochondria of Rats(Biological,Chemical)
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概要
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The effect of calcitonin (CT) on adenosine-5'-triphosphate (ATP) synthesis in the hepatic mitochondria of rats was investigated. Administration of CT (80 MRC mU/10Og body weight) produced a marked elevation of ATP content in the hepatic cytosol. This increase was completely inhibited by administration of 2,4-dinitrophenol (0.1mg/100g), an inhibitor of oxidative phosphorylation of mitochondria. Verapamil (1.0mg/100g) also inhibited the hormonal effect on hepatic ATP content. Moreover, administration of calcium chloride (2.0 and 4.0mg Ca^<2+>/100g) elevated the cytosolic ATP content by about 1.8- and 2.3-fold, respectively. Meanwhile, administration of CT produced a remarkable increase of ATPase activity in the hepatic mitochondria. Calcium contents in both. the liver and the mitochondria were raised by CT administration. The removal of the mitochondrial calcium by washing with 10mM ethyleneglycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA, pH 7.4) caused complete loss of the increase in mitochondrial adenosine triphosphatase (ATPase) activity induced by CT administration. The CT-increased ATPase activity was appreciably increased by addition of 10 and 50μM Ca^<2+>, but this increase was not altered by addition of 10μM trifluoperazine or calmodulin (2.5μg/ml). These results suggest that CT stimulates ATP synthesis in the hepatic mitochondria, and that the hormonal effect may be mediated through calcium, but not calmodulin.
- 公益社団法人日本薬学会の論文
- 1987-10-25
著者
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小川 尚美
Department Of Environmental Biochemistry Shiiuoka College Of Pharmacy
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山口 正義
Department Of Environmental Biochemistry And Toxicology School Of Pharmaceutical Sciences University
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