Effect of the Calcium-Binding Protein Regucalcin on the Ca^<2+> Transport System in Rat Liver Microsomes : The Protein Stimulates Ca^<2+> Release
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概要
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The effect of the calcium-binding protein regucalcin on the Ca^<2+> transport system in the liver microsomes from fed rats was investigated. Ca^<2+> transport was assayed by the method of Millipore filtration to estimate microsomal ^<45>Ca^<2+> accumulation following addition of 10mM adenosine triphosphate (ATP). ^<45>Ca^<2+> uptake was retarded by the presence of regucalcin (1.0-4.0μM). This retardation was remarkeble at 1min after regucalcin addition, while appreciable retardation was no longer seen at 5min. Regucalcin (2.0μM)-induced retardation of ^<45>Ca^<2+> uptake was prevented by the presence of calmodulin (5μg/ml). Calmodulin alone (1 and 5μg/ml) caused a significant increase in ^<45>Ca^<2+> uptake at 3min after the start of incubation. Also, regucalcin (2.0μM)-induced retardation of ^<45>Ca^<2+> uptake was completely blocked by the presence of a Ca^<2+> -trapping agent, oxalate (3mM). On the other hand, ^<45>Ca^<2+>, which accumulated in microsomes during 5min after ATP addition, was markedly released by the addition of regucalcin. This release was dose-dependent (0.5-4.0μM). Guanosine triphosphate (GTP; 10-100μM) caused a significant release of ^<45>Ca^<2+> from the microsomes. The presence of regucalcin (2.0μM) further enhanced the GTP effect. Regucalcin (2.0μM)-induced release of ^<45>Ca^<2+> was not blocked by the presence of the protein thiol-protecting agent dithiothretiol (0.1mM). The presence of oxalate (3mM) completely blocked the effect of regucalcin on ^<45>Ca^<2+> release from the microsomes. These results indicate that regucalcin stimulates Ca^<2+> release from liver microsomes, and that the protein retards the microsomal Ca^<2+> uptake. The present study suggests that regucalcin can regulate the Ca^<2+> transport system in rat liver microsomes.
- 公益社団法人日本薬学会の論文
- 1989-11-25
著者
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森 聖一
Department Of Environmental Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka
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山口 正義
Department of Environmental Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
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山口 正義
Department Of Environmental Biochemistry And Toxicology School Of Pharmaceutical Sciences University
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山口 正義
静岡県立大学 大学院
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山口 正義
静岡県立大学薬学部
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