Effect of Fluoride on the Activities of the Na^+/Glucose Cotransporter and Na^+/K^+-ATPase in Brush Border and Basolateral Membranes of Rat Kidney (in Vitro and in Vivo)
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概要
- 論文の詳細を見る
In this study, renal Na^+/K^+-ATPase activity was demonstrated to be strongly suppressed prior to the glucosuria caused by a fluoride dose (NaF 35mg/kg, i. p.), and the 50% suppression of the enzyme activity was almost at the same dose of NaF, about 30mg/kg, i.p. to rats. In the rats, renal Na^+/glucose cotransporter activity in brush border membranes was not affected by in vivo NaF, whereas the renal Na^+/K^+-ATPase in basolateral membranes showed a dip in activity 3h after NaF treatment of the whole animal. Moreover, it was suggested from experiments with inhibitors of calphostin C and KT5720 that protein kinase C, but not protein kinase A, may play an important role in the suppression of Na^+/K^+-ATPase following the administration of fluoride to rats. Na^+/glucose cotransporter was fairly insensitive to NaF, being competitively inhibited with a K_i of about 100 mM, whereas Na^+/K^+-ATPase was much more sensitive, with a K_i of about 2 mM. From these results, the elevation of urinary glucose excretion after a single dose of fluoride was deduced to be due to suppression of the renal Na^+/K^+-ATPase activity by a direct and/or secondary action of fluoride, rather than of the corresponding Na^+/glucose cotransporter activity.
- 社団法人日本薬学会の論文
- 1995-02-15
著者
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櫻井 孝司
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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祐田 泰延
University of Shizuoka School of Pharmaceutical Sciences, Department of Environmental Biochemistry a
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鈴木 健二
University of Shizuoka School of Pharmaceutical Sciences, Department of Environmental Biochemistry a
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滝 寿伸
University of Shizuoka School of Pharmaceutical Sciences, Department of Environmental Biochemistry a
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伊藤 康子
University of Shizuoka School of Pharmaceutical Sciences, Department of Environmental Biochemistry a
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山口 勝
University of Shizuoka School of Pharmaceutical Sciences, Department of Environmental Biochemistry a
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谷下 由紀子
University of Shizuoka School of Pharmaceutical Sciences, Department of Environmental Biochemistry a
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鈴木 健二
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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Suzuki Kenji
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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滝 寿伸
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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祐田 泰延
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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山口 勝
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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伊藤 康子
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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Suketa Yasunobu
Department Of Environmental Biochemistry And Toxicology University Of Shizuoka School Of Pharmaceuti
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谷下 由紀子
University Of Shizuoka School Of Pharmaceutical Sciences Department Of Environmental Biochemistry An
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