Spectrophotometric Determination of Phosphate Ion with Ferric Salt of Tetraphenylporphine-trisulfonic Acid
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概要
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A new indirect spectrophotometric method for the determination of phosphate ion by the use of solid ferric salt of tetraphenylporphine-trisulfonic acid (TPPSFe) was developed. The method is based on the measurement of the absorbance of TPPS liberated from TPPSFe at its Q band (procedure A) or its Soret band (procedure B). Phosphate ion ranging from 10 to 50μg and from 4 to 20μg could be determined based on the absorption coefficients of 8.2×10^3 at 512nm (procedure A) and 2.2×10^5 at 434nm (procedure B). The advantages of this method are its simple procedure and mild conditions. Procedure A was successfully applied to the determination of phosphate ion liberated from glucose-1-phosphate by acid phosphatase.
- 公益社団法人日本薬学会の論文
- 1985-08-25
著者
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斎藤 寛
Faculty of Pharmaceutical Sciences, Okayama University
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尾堂 順一
Faculty of Pharmaceutical Sciences, Okayama University
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田中 善正
Faculty of Pharmaceutical Sciences, Okayama University
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御船 正樹
Faculty of Pharmaceutical Sciences, Okayama University
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斎藤 寛
Department Of Pharmaceutical Sciences Graduate School Of Medicine And Dentistry And Pharmaceutical S
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御舩 正樹
岡山大学薬学部
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斎藤 寛
岡山大・薬
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御舩 正樹
Department Of Pharmaceutical Sciences Graduate School Of Medicine And Dentistry And Pharmaceutical S
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池上 堅治
Faculty of Pharmaceutical Sciences, Okayama University
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岡崎 公哉
Faculty of Pharmaceutical Sciences, Okayama University
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田中 善正
Faculty Of Pharmaceutical Sciences Okayama University:(present Address)department Of Biochemistry Co
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池上 堅治
Faculty Of Pharmaceutical Sciences Okayama University
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御船 正樹
Faculty Of Pharmaceutical Sciences Okayama University
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御船 正樹
Department Of Pharmaceutical Sciences Graduate School Of Medicine And Dentistry And Pharmaceutical S
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岡崎 公哉
シェリング・プラウ(株)
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田中 善正
Faculty Of Pharmaceutical Sciences Okayama University
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