Simultaneous Analysis of Genes by Capillary Electrophoresis with a Laser-Induced Fluorescence Detector Using a Stepwise Field Strength Gradient
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概要
- 論文の詳細を見る
A mixture of polymerase chain reaction (PCR) products, 100,105,300,310,485,and 500 base pair (bp) DNA fragments, was analyzed by capillary electrophoresis equipped with a laser-induced fluorescence detector (CE-LIF) using a stepwise gradient of electric field strength. The optimum condition for the analysis of PCR products was 0.5% methylcellulose and 160 V/cm from 0 to 10 min and 270 V/cm from 10 to 17 min.The length (bp) of DNA could be estimated from the relationship between the relative migration time and bp length. The relative standard deviation (R.S.D.) of DNA size (bp) was less than 3.5% and the difference from the true value was only 2.4bp.
- 公益社団法人日本薬学会の論文
- 1999-01-15
著者
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Baba Yoshinobu
Department Of Applied Chemistry Graduate School Of Engineering Nagoya University
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TSUHAKO Mitsutomo
Department of Chemistry, Kobe Pharmaceutical University
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Baba Yoshinobu
Department Of Applied Chemistry Graduate School Of Engineering Mext Innovative Research Center For P
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Baba Yoshinobu
Department Of Medicinal Chemistry Faculty Of Pharmaceutical Sciences Tokushima University
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SUMITA Chinuyo
Department of Chemistry, Kobe Pharmaceutical University
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Sumita Chinuyo
Department Of Chemistry Kobe Pharmaceutical University
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Tsuhako Mitsutomo
Department Of Chemistry Kobe Pharmaceutical University
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