Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System
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概要
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Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly (ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.
- 公益社団法人日本薬学会の論文
- 2006-04-01
著者
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Baba Yoshinobu
Department Of Applied Chemistry Graduate School Of Engineering Nagoya University
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馬場 嘉信
徳島大学薬学部薬品物理化学教室
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Baba Yoshinobu
Dep. Of Applied Chemistry Graduate School Of Engineering Nagoya Univ.
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Baba Yoshinobu
Department Of Applied Chemistry Graduate School Of Engineering Mext Innovative Research Center For P
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Kaji Noritada
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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Tokeshi Manabu
Department Of Applied Chemistry Graduate School Of Engineering Nagoya University
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Kaji Noritada
Mext Innovative Res. Center For Preventive Medical Engineering Nagoya Univ.
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JABASINI Mohammad
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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MURAKAMI Yuji
Toray Industries, Inc., New Frontiers Research Laboratories
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Murakami Yuji
Toray Industries Inc. New Frontiers Research Laboratories
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馬場 嘉信
Crest
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馬場 嘉信
徳島大学薬学部
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Jabasini Mohammad
Department Of Applied Chemistry Graduate School Of Engineering Nagoya University:toray Industries In
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Jabasini Mohammad
Department Of Medicinal Chemistry Faculty Of Pharmaceutical Sciences The University Of Tokushima Cre
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馬場 嘉信
名古屋大学大学院工学研究科
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Kaji Noritada
Department Of Applied Chemistry Graduate School Of Engineering Nagoya University
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Tokeshi Manabu
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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