Rapid Multiplexing and Simultaneous Detection of Human Spermatogenetic Failure with a 12 Lane Microchip Electrophoresis System(Analytical Biochemistry)

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概要

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• For the amplification and ultrafast separation of the genetic markers and DNA sequences that are related to human male infertility, a multiplex PCR for amplifying three DNA sequence-tagged sites (STS) located on the human Y chromosome with possible roles in the spermatogenesis process has been designed and applied followed by separation on a microchip. First, the optimum T_m degree for the three DNA markers was optimized and determined experimentally, and the three DNA STS were amplified. These three DNA markers were then separated on a 12-lane microchip electrophoresis system, which can analyze the DNA markers on 12 channels simultaneously. The combination of these two technologies, multiplex PCR and microchip electrophoresis, allows the analysis of 36 DNA markers (12×3) within only 180s.

• 公益社団法人日本薬学会の論文
• 2006-07-01

著者

• SHINKA Toshikatsu

  Department of Medical Genetics, Tohoku University School of Medicine

• NAKAHORI Yutaka

  Department of Human Genetics and Public Health, Faculty of Medicine, Graduate School of Proteomics,

• Nakahori Y

  徳島大学大学院人類遺伝学分野

• Ewis Ashraf

  National Institute Of Advanced Industrial Science And Technology (aist-shikoku) Health Technology Re

• Ewis Ashraf

  Department Of Public Health And Occupational Medicine School Of Medicine El-minia University

• Ewis Ashraf

  Health Technology Research Center National Institute Of Advanced Industrial Science And Technology (

• Baba Yoshinobu

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• 馬場 嘉信

  徳島大学薬学部薬品物理化学教室

• Baba Yoshinobu

  Dep. Of Applied Chemistry Graduate School Of Engineering Nagoya Univ.

• Baba Yoshinobu

  Department Of Applied Chemistry Graduate School Of Engineering Mext Innovative Research Center For P

• Kaji Noritada

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• Tokeshi Manabu

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• Kaji Noritada

  Mext Innovative Res. Center For Preventive Medical Engineering Nagoya Univ.

• JABASINI Mohammad

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• EWIS Ashraf

  Nanobio Research Team, Health Technology Research Center, National Institute of Advanced Industrial

• FOUAD Maged

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• DANG Fuquan

  Nanobio Research Team, Health Technology Research Center, National Institute of Advanced Industrial

• PING Guichen

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• Nakahori Yutaka

  Department Of Human Genetics And Public Health School Of Medicine University Of Tokushima

• Nakahori Y

  Univ. Tokushima Tokushima Jpn

• Nakahori Y

  Department Of Human Genetics And Public Health Faculty Of Pharmaceutical Sciences The University Of

• Nakahori Yutaka

  Department Of Human Genetics And Public Health Graduate School Of Medical Science The University Of

• Nakahori Yutaka

  Department Of Urology And Human Genetics School Of International Health University Of Tokyo

• 馬場 嘉信

  Crest

• 馬場 嘉信

  徳島大学薬学部

• Fouad Maged

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• Jabasini Mohammad

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University:toray Industries In

• Jabasini Mohammad

  Department Of Medicinal Chemistry Faculty Of Pharmaceutical Sciences The University Of Tokushima Cre

• Dang Fuquan

  Nanobio Research Team Health Technology Research Center National Institute Of Advanced Industrial Sc

• Ping Guichen

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• Shinka T

  Department Of Medical Genetics Tohoku University School Of Medicine

• 馬場 嘉信

  名古屋大学大学院工学研究科

• Kaji Noritada

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• Takahashi Shoki

  Department Of Human Genetics And Public Health Graduate School Of Medical Science University Of Tokushima

• Tokeshi Manabu

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• Shinka Toshikatsu

  Department of Human Genetics and Public Health, Graduate School of Medical Science, University of Tokushima

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