Analysis of a Variable Number of Tandem Repeats in a Heart Disease Gene by Capillary Electrophoresis with Laser-Induced Fluorescence Detector

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概要

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• Capillary electrophoresis with laser-induced fluorescence detector (CE-LIF) was successfully applied to the analysis of the variable number of tandem repeats (VNTR) in a human apolipoprotein B gene (APOB). Apolipoprotein B VNTR alleles containing more than 35 repeat units are a significant risk factor for heart disease. Thus, we developed a method for accurately determining the number of repeat units (16 bp) in the VNTR using capillary electrophoresis. The CE-LIF technique gave excellent resolution of APOB alleles differing by 2 or 4 repeat units over the range 600 to 1000 bp.The recommended conditions for the analysis of APOB VNTR loci by CE-LIF are as follows : effective length of capillary (100μm i.d., 360μm o.d.), 50cm; running buffer, 50mM Tris-borate 0.5% methylcellulose and 0.1μM fluorescent dye YO-PRO-1; electric field, 150V/cm.

• 社団法人日本薬学会の論文
• 1998-02-15

著者

• OGIHARA Toshio

  Department of Geriatric Medicine, Osaka University Graduate School of Medicine

• MIKI Tetsuro

  Department of Geriatric Medicine, Ehime University Graduate School of Medicine

• Ogihara Toshio

  Department Of Geriatric Medicine Osaka University Medical School

• Ogihara Toshio

  Department Of Geriatic Medicine

• Miki Tetsuro

  Department Of Geriatric Medicine Osaka University Medical School

• Baba Yoshinobu

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya University

• TSUHAKO Mitsutomo

  Department of Chemistry, Kobe Pharmaceutical University

• NISHIMURA Akiko

  Department of Chemistry, Kobe Pharmaceutical University

• Baba Yoshinobu

  Department Of Applied Chemistry Graduate School Of Engineering Mext Innovative Research Center For P

• Nishimura A

  Kobe Pharmaceutical Univ. Kobe Jpn

• Nishimura Akiko

  神戸薬科大学 機能性分子化

• Miki Tetsuro

  Department Of Geriatric Medicine Ehime University Graduate School Of Medicine

• Ogihara T

  Kanazawa Univ. Kanazawa Jpn

• Tsuhako M

  Department Of Functional Molecular Chemistry Kobe Pharmaceutical University

• Tsuhako Mitsutomo

  Department Of Chemistry Kobe Pharmaceutical University

• Miki Tetsuro

  Department Of Diagnostic And Therapeutic Radiology Ehime University Graduate School Of Medicine

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