Methylene Chloride Fraction of Spatholobi Caulis Induces Apoptosis via Caspase Dependent Pathway in U937 Cells(Biochemistry/Molecular Biology)
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概要
- 論文の詳細を見る
Spatholobi Caulis has been used in Oriental medicine to treat cancer and blood stasis. In this study, the methylene chloride fraction of Spatholobi Caulis (MCSC) was examined to determine if it possesses anti-cancer activity via its apoptosis-inducing activity. MCSC exhibited a strong cytotoxic effect against human monocyte leukemia U937 cells (IC_<50>=15.1μg/ml). A TUNEL assay showed that the MCSC caused a characteristic ladder pattern of discontinuous DNA fragments and apoptotic bodies. Flow cytometric analysis confirmed that MCSC significantly increases the number of apoptotic cells stained by annexin V^+/PI^- cells. Western blotting revealed that MCSC activated caspase-3 expression and cleaved poly (ADP-ribose) polymerase (PARP) in a concentration-dependent manner. An enzyme-linked immunosorbent assay (ELISA) demonstrated that MCSC significantly activated the caspase-3 activity compared with the untreated control by. Taken together, these results suggest that MCSC can induce apoptosis in U937cells via the caspase dependent pathway.
- 公益社団法人日本薬学会の論文
- 2004-09-01
著者
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LEE Eun-Ok
Department of Oncology, Graduate School of East-West Medical Science
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KIM Sung-Hoon
Department of Oncology, Graduate School of East-West Medical Science
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Kim J‐h
Korea Res. Inst. Of Bioscience And Biotechnology Daejeon Kor
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Lim Nak-cheol
Department Of Oriental Pathology And Internal Medicine Oriental Medical College Daejeon University
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Lee Eun-ok
Department Of Oncology Graduate School Of East-west Medical Science
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Lee Eun-ok
Department Of Oncoloev Graduate School Of East-west Medical Science Kyunghee University
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Kim Sung-hoon
Department Of Oncoloev Graduate School Of East-west Medical Science Kyunghee University
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Park Jong-oh
Department Of Electronics Engineering Dong-a Univ.
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Yoon Taek-joon
Department Of Oncoloev Graduate School Of East-west Medical Science Kyunghee University
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Yoon Taek-joon
Department Of Biotechnology College Of Biomedical And Health Science Konkuk University
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Park Jong-oh
Department Of Oriental Pathology And Internal Medicine Oriental Medical College Daejeon University
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Jung Sung-ki
Department Of Internal Medicine College Of Oriental Medicine Kyunghee University
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Kim Sung-hoon
Department Of Chemistry Konkuk University
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HA Eun-Suk
Department of Oncoloev, Graduate School of East-West Medical Science, Kyunghee University
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KIM Jin-Hyung
Department of Oncoloev, Graduate School of East-West Medical Science, Kyunghee University
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YOON Byung-Soo
Department of Biology, College of Natural Science and Engineering, Kyonggi University
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Ha Eun-suk
Department Of Oncoloev Graduate School Of East-west Medical Science Kyunghee University
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Kim Jin-hyung
Department Of Computer Science Kaist
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Kim Jin-hyung
Department Of Oncoloev Graduate School Of East-west Medical Science Kyunghee University
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Yoon Byung-soo
Department Of Biology College Of Natural Science And Engineering Kyonggi University
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Lee Eun-Ok
Department of Chemical Engineering and Bioprocess Engineering Research Centre, Korea Advanced Institute of Science and Technology
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