Specific Increase in the Number of Vanadium-Containing Blood Cells by Some Ionophores and Inhibitors of Proton-ATPases in the Ascidian, Ascidia sydneiensis samea
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概要
- 論文の詳細を見る
Ascidians are known to accumulate high levels of vanadium. Vanadium-accumulat8ng blood cells (vanadocytes) have one large and highly acidic vacuole. Recently, it was found unexpectedly that the number of vanadocytes increased rapidly and significantly when ascidians were immersed in 10 mM or 20 mM NH_4Cl solution for 20 hr to neutralize vacuole content. Suspecting that changes in intra-organellar pH and in levels of ATP caused by the treatment might be involved, we examined whether or not several reagents that perturb either acidic pH or ATP synthesis affected the increase in the number of vanadocytes. SF6847 (a proton conductor), nigericin, monensin, valinomycin (ionophores), 2, 4-dinitrophenol (an uncoupler), bafilomycin A_1 (a V-ATPase inhibitor), oligomycin and NaN_3 (F-ATPase inhibitors) all increased the number of vanadocytes by about three-to five-fold over that of control. However, treatment with NaCl, KCl, LiCl, CaCl_2, TJ24373, sporeamycin (macrolide antibiotics), ouabain and Na_3VO_4 (P-ATPase inhibitors) had no effect on the increase. These results suggest that neutralization of intra-organellar pH triggers an increase in the number of vanadocytes. Vanadocytes that increased in number in the coelomic fluid after treatment were revealed by immunohistochemical study, to have originated in the connective tissues around the alimentary canal.
- 社団法人日本動物学会の論文
著者
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Uyama Taro
Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog
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Uyama T.
Marine Biological Laboratory Graduate School Of Science Hiroshima University
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Moriyama Yoshinori
Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog
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Moriyama Yoshinori
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Kanazawa University:(present Address)
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Moriyama Yoshinori
Marine Biological Laboratory Graduate Department Of Gene Science Faculty Of Sciences Hiroshima Unive
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Nose Yasuhiro
Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog
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Hayashi Mitsuko
Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog
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Michibata Hitoshi
Mukaishima Marine Biological Laboratory, Faculty of Science and Laboratory of Marine Molecular Biolo
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Michibata Hitoshi
Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog
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