Isolation of cDNAs Encoding Subunits A and B of the Vacuolar-Type ATPase from the Vanadium-Rich Ascidian, Ascidia sydneiensis samea(Physiology)

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概要

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• Vacuolar-type H^+-ATPases (V-ATPases), which are composed of at least ten different subunits, can generate a proton-motive force by hydrolyzing ATP and acidify the contents of various intracellular organelles. Subunits A and B of V-ATPase have been detected immunologically in ascidian blood cells, predominantly in signet ring cells (vanadocytes), which accumulate vanadium in their vacuoles. The action of V-ATPase in ascidian blood cells has been demonstrated by the fact that bafilomycin A^1, a specific inhibitor of V-ATPases, inhibits the acidification of the vacuoles of vanadocytes. As the next step in studying the function of V-ATPase in vanadocytes, we isolated cDNAs encoding subunits A and B of V-ATPase from the blood cells of an ascidian, Ascidia sydneiensis samea. The nucleotide sequences of the cDNAs for subunits A and B encoded proteins of 619 and 509 amino acids, respectively, both of which were highly conserved among organisms.

• 社団法人日本動物学会の論文
• 1998-12-15

著者

• Uyama Taro

  Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog

• Uyama T.

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• 金森 寛

  Marine Biotechnology Institute

• KANAMORI Kan

  Department of Chemistry,Faculty of Science,Toyama University

• Kanamori Kan

  Department Of Chemistry Faculty Of Science Toyama University

• Kanamori K

  Marine Biotechnology Institute

• Ueki T

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

• Ueki Tatsuya

  Mukaishima Marine Biological Laboratory, Faculty of Science and Laboratory of Marine Molecular Biolo

• Michibata Hitoshi

  Mukaishima Marine Biological Laboratory, Faculty of Science and Laboratory of Marine Molecular Biolo

• Michibata Hitoshi

  Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog

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