Expressed Sequence Tag Analysis of Blood Cells in the Vanadium-Rich Ascidian, Ascidia sydneiensis samea : A Survey of Genes for Metal Accumulation(Physiology)

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概要

• 論文の詳細を見る

• Some species in the family Ascidiidae accumulate vanadium at concentrations in excess of 350 mM, which corresponds to about 10^7 times that found in seawater. The vanadium ions are stored in vacuoles located within vanadium-containing blood cells, vanadocytes. To investigate the phenomenon, an expressed sequence tag analysis (EST) of a cDNA library of Ascidia sydneiensis samea blood cells was carried out. Three hundred clones were obtained and sequenced by EST analysis. A similarity search revealed that 158 of the clones (52.7%) were known genes, and 142 of the clones (47.3%) did not have any similarity to genes registered in the SwissProt database. According to the functions of their genes the identified EST clones were categorized into eight types of clones; these consisted of genes; metal-related proteins (29 clones), signal transduction (22 clones), protein synthesis (17 clones), nuclear proteins (17 clones), cytoskeleton and motility (14 clones), energy conversion (3 clones), hypothetical proteins (11 clones), and others (45 clones). The ferritin homologue has a high degree of similarity to that of mammals; the iron-binding sites of ferritin are well conserved including His-118 which is important for capturing Fe^<2+>, also works as a ligand for VO^<2+>.

• 社団法人日本動物学会の論文
• 2002-09-25

著者

• Uyama T

  Hiroshima Univ. Hiroshima Jpn

• Uyama Taro

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Uyama Taro

  Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog

• Uyama Taro

  Marine Biol. Lab. Grad. Sci. Sch. Hiroshima Univ

• Uyama T.

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Yamaguchi N.

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Michibata H

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

• Michibata Hitoshi

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Yamaguchi Nobuo

  Marine Biological Laboratory, Graduate School of Science, Hiroshima University

• Togi Akiko

  Minase Research Institute, Sekisui Chemical Co., Ltd.

• Ueki Tatsuya

  Minase Research Institute, Sekisui Chemical Co., Ltd.

• Togi A

  Minase Research Institute Sekisui Chemical Co. Ltd.

• Ueki T

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

• Yamaguchi N

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Ueki Tatsuya

  Department Of Zoology Graduate School Of Science Kyoto University:(present Address)department Of Mor

• Yamaguchi Nobuo

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Ueki T

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

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