A 100-kDa Antigen Recognized by a Newly Prepared Monoclonal Antibody Specific to the Vanadocytes of the Vanadium-Rich Ascidian, Ascidia sydneiensis samea, is Glycogen Phosphorylase(Physiology)

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概要

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• Ascidians have the unusual physiological ability to accumulate high levels of vanadium and reduce it to the +3 oxidation state (V^<lll>) in vanadocytes, the vanadium-containing blood cells. We are characterizing several polypeptides specific to vanadocytes that may participate in this. This study revealed that a 100-kDa antigen, recognized by a newly prepared monoclonal antibody, S8E4, is exclusively localized in vanadocytes, and identified the antigen as glycogen phosphorylase (EC 2.4.1.1) by sequencing the encoded cDNA. Since two enzymes, glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (EC 1.1.1.44), both in the pentose phosphate pathway, have already been identified in vanadocytes, at least three enzymes involved in carbohydrate metabolism are localized in vanadocytes in huge amounts.

• 社団法人日本動物学会の論文
• 1998-12-15

著者

• Uyama Taro

  Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog

• Uyama T.

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• 金森 寛

  Marine Biotechnology Institute

• KANAMORI Kan

  Department of Chemistry,Faculty of Science,Toyama University

• Kanamori Kan

  Department Of Chemistry Faculty Of Science Toyama University

• Kanamori K

  Marine Biotechnology Institute

• Ueki T

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

• Ueki Tatsuya

  Mukaishima Marine Biological Laboratory, Faculty of Science and Laboratory of Marine Molecular Biolo

• Michibata Hitoshi

  Mukaishima Marine Biological Laboratory, Faculty of Science and Laboratory of Marine Molecular Biolo

• Suhama Yoshitake

  Mukaishima Marine Biological Laboratory, Faculty of Science, and Laboratory of Marine Molecular Biol

• Michibata Hitoshi

  Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog

• Suhama Yoshitake

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

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