Subunit C of the Vacuolar-Type ATPase from the Vanadium-Rich Ascidian, Ascidia sydneiensis samea, Rescued the pH Sensitivity of Yeast vma5 Mutants

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概要

• 論文の詳細を見る

• A vanadium-accumulating ascidian, Ascidia sydneiensis samea, expresses vacuolar-type H+-ATPases (V ATPases) on the vacuole membrane of the vanadium-containing blood cells known as vanadocytes. Previously, we showed that the contents of their vacuoles are extremely acidic and that a V ATPase-specific inhibitor, bafilomycin A1, neutralized the contents of the vacuoles. To understand the function of V ATPase in vanadocytes, we isolated cDNA encoding subunit C of V ATPase from vanadocytes since this subunit has been known to be responsible for the assembly of V-ATPases and to regulate the ATPase activity of V-ATPases. The cloned cDNA was 1,443 nucleotides in length, and encoded a putative 384 amino-acid protein. By expressing the ascidian cDNA for subunit C under the control of a galactose-inducible promoter, the pH-sensitive phenotype of the corresponding vma5 mutant of a budding yeast was rescued. This result showed that the ascidian cDNA for subunit C functioned in yeast cells.

• Springerの論文
• 2001-07-01

著者

• Uyama T

  Hiroshima Univ. Hiroshima Jpn

• Uyama Taro

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Uyama Taro

  Mukaishima Marine Biological Laboratory Faculty Of Science And Laboratory Of Marine Molecular Biolog

• Uyama Taro

  Marine Biol. Lab. Grad. Sci. Sch. Hiroshima Univ

• Uyama T.

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• 金森 寛

  Marine Biotechnology Institute

• Michibata Hitoshi

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• KANAMORI Kan

  Department of Chemistry,Faculty of Science,Toyama University

• Kanamori Kan

  Department Of Chemistry Faculty Of Science Toyama University

• UEKI Tatsuya

  Marine Biological Laboratory, Graduate School of Science, Hiroshima University

• Kanamori K

  Marine Biotechnology Institute

• Ueki T

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

• Ueki Tatsuya

  Marine Biological Laboratory Graduate School Of Science Hiroshima University

• Ueki T

  Molecular Physiology Laboratory Department Of Biological Science Graduate School Of Science Hiroshim

• 植木 龍也

  Marine Biological Laboratory, Graduate School of Science, Hiroshima University

• ウエキ タツヤ

  Department of Chemistry, Faculty of Science, Toyama University

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