Increased Expression of Cofilin in Denervated Chicken Skeletal Muscle

概要

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Neonatal or adult chicken fast pectoralis (breast) muscle (PM) was denervated and at 2-8 weeks after the operation, the cofilin expression was examined by three different methods ; immunoblotting, immunocytochemistry and Northern RNA blotting. Since cofilin expression is down-regulated during normal postnatal development of PM (Abe et al., J. Biochem. 106: 696-702,1989), cofilin was detected in the adult muscle only in a small amount. When PM of neonatal chicken was denervated at 2 days after hatching, the amount of cofilin in the muscle was maintained at a high level through postnatal development. In the case of adult muscle denervation, cofilin expression was remarkably increased in 2 months after denervation. In order to localize cofilin in individual muscle fibers, the cryosections of the denervated muscle were examined with anti-cofilin antibody (MAB-22). The antibody stained the majority of cells of different size, indicating that cofilin expression was induced in the regenerating muscle cells as well as in the pre-existing myofibers. We suggest that cofilin is involved in reorganization of actin in the denervated muscle.
社団法人日本動物学会の論文
1993-08-16