QUANTITATIVE IN VITRO AUTORADIOGRAPHY OF NEUROTRANSMITTER RECEPTORS : APPLICATIONS TO DISEASED HUMAN BRAINS
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概要
- 論文の詳細を見る
The technique of in vitro autoradiography is widely utilized for observations of specific binding sites of neurotransmitters or drugs. The current trend of this research field is toward quantifying receptor densities in autoradiograms of brain sections. In this paper, the authors showed the method for quantification in our laboratory and confirmed that receptor densities obtained from in vitro autoradiography almost consisted with those from receptor binding assay. Since in vitro autoradiography can construct highresolution maps of receptor distributions, it is the useful tool for studies of neural functions. The authors performed the quantitative in vitro autoradiography in diseased human brains. Two cases of late cortical cerebellar atrophy (LCCA) were used in these experiments. Through morphometric analysis, it was observed that Purkinje cells in both case 1 and 2 were lost in half as many as those in control cases and the density of the granular cells was markedly reduced in the cerebellar cortex of case 2. In the dentate nucleus of case 1, the increase of GABA receptors as well as the decrease of GABA concentration was observed and this increase is considered to be receptor supersensitivity. In addition, the reduction of glutamate binding sites was observed in the dentate nucleus of both cases. Neurobiological basis of this receptor change is left for further investigations.
- 日本組織細胞化学会の論文
著者
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KITO Shozo
Third Department of Internal Medicine, Hiroshima University, School of Medicine
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Kito Shozo
Third Department Of Internal Medicine Hiroshima University School Of Medicine:department Of Neurolog
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Kito Shozo
Third Department Of Internal Medicine Hiroshima University
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Kanazawa Ichiro
Third Department Of Internal Medicine Hiroshima University School Of Medicine:department Of Neurolog
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Kanazawa Ichiro
Department Of Degenerative Neurological Diseases National Institute Of Neuroscience National Center
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Matsubayashi Hiroaki
Department Of Molecular And Pharmacological Neuroscience Graduate School Of Biomedical Sciences Hiro
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Matsubayashi Hiroaki
Third Department Of Internal Medicine Hiroshima University School Of Medicine:department Of Neurolog
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MIYOSHI Rie
Third Department of Internal Medicine, Hiroshima University School of Medicine
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Miyoshi Rie
Third Department Of Internal Medicine Hiroshima University School Of Medicine:department Of Neurolog
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Matsubayashi Hiroaki
Department Of Ophthalmology And Visual Sciences Graduate School Of Biomedical Sciences Hiroshima Uni
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