Quantitative autoradiographic localization of the M1 and M2 subtypes of muscarinic acetylcholine receptors in the monkey brain.
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概要
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The distribution of muscarinic acetylcholine receptors (mAChR) was investigated in the monkey brain by means of quantitative in vitro autoradiography. <SUP>3</SUP>H-QNB, <SUP>3</SUP>H-pirenzepine (PZ) and <SUP>3</SUP>H-AF-DX 116 were used for labelling total mAChR, M<SUB>1</SUB> and M<SUB>2</SUB> receptors, respectively. <SUP>3</SUP>H-PZ and <SUP>3</SUP>H-AF-DX 116 showed specificity to each receptor subtype in the monkey brain. On sections containing the putamen and giobus pallidus, the sum of B<SUB>max</SUB> values of <SUP>3</SUP>H-PZ and <SUP>3</SUP>H-AF-DX 116 binding sites was almost close to that of <SUP>3</SUP>H-QNB binding sites. Autoradiographic distributions of muscarinic subtype receptors in the monkey brain were similar to those reported in the rat brain; that is, M<SUB>1</SUB> receptors were dominant in most areas of the telencephalon, while M<SUB>2</SUB> receptors were richly distributed in the brainstem and cerebellum. However, some nuclei of the brainstem such as the central gray matter, superior colliculus, substantia nigra, nucleus of the oculomotor nerve, pontine nucleus and inferior olivary nucleus, had relatively high ratios of M<SUB>1</SUB> receptors in the monkey brain. In addition, the cortical lamminar distribution of M<SUB>2</SUB> receptors noticed in the rat was not observed in the monkey brain. Knowledge about the localizations of M<SUB>1</SUB> and M<SUB>2</SUB> receptors in various brain regions in the monkey brain will increase our understanding of the functions of the brain cholinergic system in the primate.
- 公益社団法人 日本薬理学会の論文
著者
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Kito Shozo
Third Department Of Internal Medicine Hiroshima University
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Miyoshi Rie
Third Department Of Internal Medicine Hiroshima University School Of Medicine:department Of Neurolog
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SHIMOYAMA Masanori
Third Department of Internal Medicine, Hiroshima University School of Medicine
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