IMMUNOCYTOCHEMICAL EVIDENCE OF NEURON SPECIFIC ENOLASE (NSE) IN HUMAN ADENOVIRUS 12-INDUCED RETINOBLASTOMA-LIKE TUMOR CELLS IN VITRO
スポンサーリンク
概要
- 論文の詳細を見る
Retinoblastoma-like tumor cells (EXP-5 cell line) derived from a tumor in an inbred albino rat by human adenovirus 12 have been maintained in culture. We investigated inimunocytochemical localization of neuron specific enolase (NSE) and S-100 protein in cultured tumor cells before and after successful differentiation into neuronal and glial-like cells. After the addition of cyclic AMP analog, a neurite-like process developed, and they tended to form a network after 24 hr. Before and after successful differentiation, the cultured cells exhibited simultaneous expression of NSE and S-100 protein. Differentiated cells showed a tendency to express the marker that correlated with their morphology. These results supported the idea that adenovirus 12-induced retinoblastoma-like tumor was derived from bipotential precurcer cells.
- 日本組織細胞化学会の論文
著者
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SAWADA Tatsuo
Department of Mechanical Engineering, Keio University
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Kobayashi Makio
Department of Pathology, Tokyo Women’s Medical University
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Kobayashi Makio
Department Of Pathology Yamanashi Medical College Yamanashi And Nihon University School Of Medicine
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Sawada Tatsuo
Department Of Mechanical Engineering Keio University
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Sawada Tatsuo
Department Of Pathology Yamanashi Medical College Yamanashi And Nihon University School Of Medicine
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MUKAI NORITSUGU
Experimental Neuropathology Laboratory, Eye Research Institute of Retina Foundation, and Department
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Kobayashi Makio
Department Of Immunology And Pathology Yamanashi Medical College
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Mukai Noritsugu
Experimental Neuropathology Laboratory Eye Research Institute Of Retina Foundation And Department Of
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Kobayashi Makio
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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