Immunohistochemical Detection of 13(R)-hydroxyoctadecadienoic Acid in Atherosclerotic Plaques of Human Carotid Arteries Using a Novel Specific Antibody
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概要
- 論文の詳細を見る
13-Hydroxyoctadecadienoic acid (13-HODE) is a major component of oxidized low density lipoprotein (OxLDL), which has been shown to have a crucial role in atherogenesis. Of the 13-HODE stereoisomers, 13(S)-HODE and 13(R)-HODE, little is known about the latter in contrast to the former. To detect 13(R)-HODE in atherosclerotic lesions, we prepared a mouse monoclonal antibody against 13(R)-HODE. Competitive enzyme-linked immunosorbent assay clarified the selective reaction of a clone mAb 13H1 with both free and bovine serum albumin-conjugated forms of 13(R)-HODE but not other oxidized lipids including 13(S)-HODE. Immunohistochemical analysis revealed the colocalization of 13(R)-HODE immunoreactivity with the OxLDL marker oxidized phophatidylcholine immunoreactivity in vascular endothelial cells, macrophages and migrating vascular smooth muscle cells in atherosclerotic plaques of human carotid arteries. The present results provide in vivo evidence for the formation of 13(R)-HODE in atherosclerotic lesions of carotid arteries.
著者
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Itabe Hiroyuki
Department Of Biological Chemistry School Of Pharmaceutical Sciences Showa University
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Kawamata Takakazu
Department Of Neurosurgery Institute Of Clinical Neurology Tokyo Women's Medical University
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Okada Yoshikazu
Department Of Medical Informatics Tokai University School Of Medicine
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Toi Sono
Department Of Cardiology The University Of Texas M. D. Anderson Cancer Center
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Uchida Koji
Laboratory Of Food And Biodynam Ics Department Of Applied Biological Sciences Nagoya University
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Sawada Tatsuo
Department Of Mechanical Engineering Keio University
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Kobayashi Makio
Department Of Immunology And Pathology Yamanashi Medical College
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Shibata Noriyuki
Department Of Pathology School Of Medicine Tokyo Women's Medical University
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Sawada Tatsuo
Department of Pathology, Tokyo Women's Medical University
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Uchiyama Shinichiro
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University
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Uchida Koji
Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences
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Shibata Takahiro
Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences
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Kobayashi Makio
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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Kawamata Takakazu
Department of Neurosurgery, Yachiyo Medical Center
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