Long-Chain Fatty Acids Induce Lipid Droplet Formation in a Cultured Human Hepatocyte in a Manner Dependent of Acyl-CoA Synthetase(Highlighted paper selected by Editor-in-chief,Molecular and Cell Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Lipid droplets (LDs) are intracellular storage sites of neutral lipids, which accumulate in fatty liver disease. Here, we investigated the effects of fatty acids and glucose on LD formation in a cultured human hepatocyte, HuH7, by adding them to culture media. Fatty acids with carbohydrate chains C12-C18 efficiently induced LDs, but those of C8 and C10 were ineffective. Glucose did not induce LD formation even in the presence of insulin. Oleic acid induced significant increases in cellular neutral lipids, and cell fractionation revealed that most of the newly synthesized neutral lipids were concentrated in LDs together with LD proteins. The LD formation was not abrogated by removal of medium glucose but was significantly inhibited by an ACSL inhibitor, triacsin C. These results demonstrate that long-chain fatty acids contribute to LD formation to a greater extent than glucose, possibly by being taken up into the cells, activated by ACSL, reconstituted into neutral lipids and then stored in LDs. Pregnenolone and lithium did not suppress oleic acid-dependent LD formation, despite previous reports of their ability to inhibit LD formation in macrophages and adipocytes suggesting differences among LD formations in these cells.
- 公益社団法人日本薬学会の論文
- 2006-11-01
著者
-
Takano Tatsuya
帝京大学薬学部病態生化学教室
-
高野 達哉
帝京大薬
-
板部 洋之
帝京大学薬学部病態生化学教室
-
板部 洋之
昭和大薬・生物化学
-
板部 洋之
帝京大学薬学部微生物・病態生化学教室
-
Morita M
Department Of Biological Chemistry Graduate School Of Medicine & Pharmaceutical Sciences Univers
-
Morita Masashi
富山大学 医学薬学研究分子細胞機能
-
TAKANO Tatsuya
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo Unive
-
高野 達哉
帝京大学薬学部微生物・病態生化学教室
-
HOMMA Koichi
Department of Communication and Network Engineering, Tokai University
-
MORI Masahiro
Department of Internal Medicine , Keio University
-
Itabe Hiroyuki
Department Of Biological Chemistry School Of Pharmaceutical Sciences Showa University
-
Fujimoto Yasuyuki
Department of Molecular Biology, School of Pharmacy, Iwate Medical University
-
高野 達哉
帝京大学薬学部医療薬学
-
Higashi Yusuke
Section Of Cardiology Department Of Medicine Tulane University Health Sciences Center
-
ONODUKA Jun
Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
YAMAGUCHI Shinji
Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
MAKITA Minoru
Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
KINOSHITA Tetsuaki
Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
NODA Jun-ichi
Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
-
Yamaguchi Shinji
Department Of Electrical Engineering Suzuka Technical College
-
Homma Koichi
Department Of Communication And Network Engineering Tokai University
-
Homma Koichi
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Takano Tatsuya
Department Of Microbiology And Molecular Pathology Faculty Of Phamaceutical Sciences Teikyo Universi
-
Onoduka Jun
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Noda Jun-ichi
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Makita Minoru
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Yasuyuki Fujimoto
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Yamaguchi Shinji
Department Of Earth Sciences And Technology Akita University
-
Yamaguchi Shinji
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Yamaguchi Shinji
Department Of Earth Science And Technology Faculty Of Engineering And Reosurce Science Akita Univers
-
Mori Masahiro
Department Of Cancer Research Research Institute For Nuclear Medicine And Biology Hiroshima Universi
-
Mori Masahiro
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Kinoshita Tetsuaki
Department Of Molecular Pathology Faculty Of Pharmaceutical Sciences Teikyo University
-
Homma Koichi
Department of Applied Chemistry, Faculty of Science, Science University of Tokyo
関連論文
- Pilot Signal Generation Scheme Using Frequency-Dependent Cyclic Shift ZC Sequence for Inter-Cell Interference Mitigation
- P552 不安定狭心症の種々の病型における血漿中酸化LDL値の差異
- P065 急性心筋梗塞症の慢性期における血漿中酸化LDL値の推移と心事故発生との関連性
- 0417 マクロファージの酸化LDL取り込みと細胞内酸化PC-apoB複合体蓄積
- SI-7 ヒト冠動脈のプラーク不安定化における酸化LDLおよびレクチン様酸化LDL受容体(LOX-1)の関与
- ヒト冠動脈における Plaque Vulnerability と酸化LDLの意義
- P803 ヒト冠動脈のプラーク進展・変貌過程における酸化phosphatidylcholineとapolipoproteinBの関与
- 動脈硬化の分子医学 -司会の言葉-
- 核小体型PHGPxは新規細胞増殖抑制因子である
- PC-cholesterol complex の形成と泡沫細胞崩壊に伴う過酸化脂質の蓄積
- FC-rich 脂質粒の形成と泡沫細胞崩壊機構
- ヒト動脈硬化病巣表層部を認識するモノクロナル抗体の抗原解析
- 動脈硬化におけるCholesterolの蓄積 : 粥腫形成・進展機構の分子病理学的解析
- 泡沫細胞脂肪滴局在タンパク質ADRPの誘導・分解とその役割
- 脂肪滴は脂質代謝酵素に富んだ細胞内構造体である
- 17β-hydroxysteroid dehydrogenase 11 は細胞内脂質顆粒に局在する
- P022 虚血性心疾患における血漿中酸化LDL値と冠疾患危険因子との関連性
- ラット肝ペルオキシソームに存在する酸化タンパク質の同定とその分解機構
- 0600 急性心筋梗塞症における血漿酸化LDL値の推移について
- P344 急性冠動脈症候群における血漿中酸化LDLと炎症指標c反応蛋白との関連性についての研究
- P280 冠動脈アテレクトミーと冠動脈内膜剥離術より得られた組織標本における酸化LDL局在の意義について
- 0323 急性冠動脈症候群における血漿中酸化LDLの意義について
- 肝脂質蓄積時に現れる中間比重脂肪滴の生成機構
- Involvement of von Willebrand Factor and PGI_2 in Platelet Binding to a Partially Denuded Endothelial Monolayer
- 血漿中の酸化PCはLCATによる代謝を受けている
- 腸上皮細胞株CaCo-2におけるGPI結合型アルカリフォスファターゼの極性輸送とカベオリン
- 酸化LDLにおける酸化リン脂質による蛋白質の修飾
- 動脈硬化粥腫認識モノクローナル抗体とその抗原解析
- 泡沫細胞を認識する抗酸化LDLモノクローナル抗体
- Lysosomeの生化学--その最近の進歩
- 司会のことば
- 0758 ヒト冠動脈硬化病変の初期進展過程における酸化LDLの関与
- P281 酸化LDL陽性粥状動脈硬化病変の病理組織学的特徴 : Directional coronary atherectomy切除標本による検討
- Clinical and Experimental Studies on Pulmonary and Coronary Circulation : PROCEEDINGS OF THE 25TH ANNUAL MEETING JAPANESE CIRCULATION SOCIETY (Part II)
- 学術振興賞受賞 板部洋之氏の業績
- Insulin-Degrading Enzyme Exists Inside of Rat Liver Peroxisomes and Degrades Oxidized Proteins
- Metabolism of Oxidized Phosphatidylcholines Formed in Oxidized Low Density Lipoprotein by Lecithin-Cholesterol Acyltransferase^1
- 酸化LDLの修飾構造と代謝について 1
- Characterization of Two Chinese Hamster Ovary Cell Lines Expressing the COOH-terminal Domains of Sterol Regulatory Element-binding Protein (SREBP)-1
- 薬学教育モデルカリキュラム作成に当っての考え方 : 薬学教育、薬剤師教育に関する基本姿勢
- Impurity Behavior during the H-Mode in JFT-2M
- Ion Bernstein Wave Experiment on JFT-2M Tokamak
- Enhancement of Wall Fueling and Improvement in nτ of a Beam-Heated H-Mode Discharge by Electron Cyclotron Wave in the JFT-2M Tokamak
- Measurement of Peripheral Electron Temperature by Electron Cyclotron Emission during the H-Mode Transition in JFT-2M Tokamak
- リソゾ-ムの動向 (動脈硬化症--臨床の進歩) -- (動脈硬化症の病態)
- 動脈硬化研究のめたの分子病理学
- 座長のあり方について(年会一般講演の座長のあり方)
- 生体材料人工血管の開発を目的とした血管平滑細胞の3次元collagen gel内phenotype変換に関する研究
- SB4 粥状動脈硬化症とMacrophage : 細胞化学ならびに生化学的立場から
- W-2-4 大学院臨床コース「病棟実習」におけるプレ病棟教育導入の試み (1) : コミュニケーション実習、TDM 実習、模擬症例検討会の効果
- Tumor Induction in B6C3F1 Mice by Californium-252 1,2,3
- Carotid endarterectomy (CEA) 前後における頸部頸動脈超音波検査の意義
- Magnetic Excitations in the Spin Gap System KCuCl_3
- ATP-Binding and -Hydrolysis Activities of ALDP (ABCD1) and ALDRP (ABCD2), Human Peroxisomal ABC Proteins, Overexpressed in Sf21 Cells(Molecular and Cell Biology)
- Optimization Studies on the Plasma Position Control System in TORIUT-4 Tokamak
- Computer Simulation of Ion Beam Extraction by Finite Element Method
- Prevalence and mechanism of urinary dysfunction in Guillain-Barre syndrome
- Plasma oxidized low density lipoprotein levels in patients with coronary artery disease, gastritis with Helicobacter pylori infection, inflammatory bowel disease
- P-457 虚血再潅流障害肝における酸化LDLの発現と局在
- 1. 動脈硬化と酸化ストレス(L2-A 動脈硬化の研究と臨床最前線)
- 酸化LDLの生成と代謝
- モノクローナル抗体を用いた酸化LDLの高感度定量と動脈硬化
- 生活習慣病と酸化ストレス(生活習慣病)
- 酸化 LDL を基盤とした動脈硬化研究の展開 酸化 LDL 中の酸化リン脂質とその意義^1
- 変性リポタンパク質と動脈硬化
- Pilot Signal Generation Scheme Using Frequency-Dependent Cyclic Shift ZC Sequence for Inter-Cell Interference Mitigation
- Study of Runaway Electron Transport in Edge Stochastic Magnetic Field in the JFT-2M Tokamak
- Chromosomal Polymorphism of Two Pickling Cucumbers (Cucumis sativus L.) Revealed by Fluorescent Staining with CMA and DAPI
- Chromosomal polymorphism in cucumber cultivar (Cucumis sativus L.) revealed by fluorescence staining and FISH
- Mutation Study of Antithrombin : The Roles of Disulfide Bonds in Intracellular Accumulation and Formation of Russell Body-Like Structures
- β-TUBULIN ISOTYPES IN TOBACCO
- Isotype-specific Changes in the Amount of β-Tubulin RNA in Synchronized Tobacco BY2 Cells
- Characterization of Vitronectins in Atherosclerotic Lesions
- Long-Chain Fatty Acids Induce Lipid Droplet Formation in a Cultured Human Hepatocyte in a Manner Dependent of Acyl-CoA Synthetase(Highlighted paper selected by Editor-in-chief,Molecular and Cell Biology)
- Minimally Modified LDL Is an Oxidized LDL Enriched with Oxidized Phosphatidylcholines
- ATP-dependent Lysis of Isolated Lysosomes by Basic Substances and Acidic Ionophores
- 動脈硬化の成因を探る : 粥状硬化巣を認識する単クローン抗体の作製と応用
- 内皮細胞の物質輸送 (内皮細胞をさぐる)
- 内皮細胞シリ-ズ-2-内皮細胞と物質透過
- 動脈硬化成因の分子病理学
- 培養内皮細胞を介した物質の透過機構
- INVOLVEMENT OF MACROPHAGES IN ACCUMULATION AND ELIMINATION OF CHOLESTEROL ESTER IN ATHEROSCLEROTIC AORTA (Cytochemistry of Macrophage)
- ライソゾ-ム(みにれびゅう)
- Magnetic and Electrical Properties of a Single-Grained Al_Pd_Mn_9 Icosahedral Quasicrystal
- Dynamical Diffraction Effect on Fluorescent X-Ray Emission in Absorbing Perfect Germanium Crystals in the Laue Geometry
- Dynamical Diffraction Effect on X-Ray Inelastic Scattering in Absorbing Perfect Germanium Crystal in the Laue Geometry
- Hydrolysis of Lipid Droplets in Acid Cholesteryl-Esterase-Deficient Fibroblasts
- Field Experiments on 16QAM/TDMA and Trellis Coded 16QAM/TDMA Systems for Digital Land Mobile Radio Communications (Special Issue on Personal, Indoor and Mobile Radio Communications)
- Experimental Performance Results of Coherent Wideband DS-CDMA with TDD Scheme(Special Issue on ThirdGeneration Land Mobile Communication Systems)
- Wideband CDMA Systems in TDD-Mode Operation for IMT-2000(Special Issue on Third Generation Land Mobile Communication Systems)
- Comparative Evaluation between Parallel Combinatory CDMA and Direct Sequence CDMA for High Speed Radio Communication(Special Section on Fundamentals of Multi-dimensional Mobile Information Network)
- Novel Benzoxazole 2,4-Thiazolidinediones as Potent Hypoglycemic Agents. Synthesis and Structure-Activity Relationships
- CINEMICROPHOTOGRAPHIC OBSERVATION OF AORTIC FOAM CELLS CONTAINING ANISOTROPIC LIPID INCLUSIONS
- LYSOSOMAL H^+- ADENOSINE TRIPHOSPHATASE IS SIMILAR TO MITOCHONDRIAL H^+- ADENOSINE TRIPHOSPHATASE IN ITS SENSITIVITY TO DRUGS AND ANIONS AFTER SOLUBILIZATION
- Synthesis of 1,2,3,4-Tetrahydro-β-carboline Derivatives as Hepatoprotective Agents. III. : Introduction of Substituents onto Methyl 1,2,3,4-Tetrahydro-β-carboline-2-carbodithioate(Medeicinal Chemistry,Chemical)
- DS-CDMA System with Symbol Ranking Type Interference Canceller(SRIC)(Special Issue on Third Generation Land Mobile Communication Systems)
- Soft Decision Viterbi Decoding and Self-Interference Cancellation for High Speed Radio Communication by Parallel Combinatory CDMA (Special Section on Mutli-dimensional Mobile Information Network)
- 酸化リポ蛋白質と病態 (膜脂質の過酸化)
- モノクロ-ナル抗体を用いた生体過酸化脂質の測定 (プロシ-ディングス(生体内脂質の過酸化--測定法と正常値))
- 酸化ホスファチジルコリンと動脈硬化