SENSITIVE IMMUNOHISTOCHEMICAL DEMONSTRATION OF ENDOGENOUS PROLACTIN IN MAMMARY GLAND AND DMBA-INDUCED MAMMARY CARCINOMA OF RATS BY ABC METHOD
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概要
- 論文の詳細を見る
Endogenous immunoreactive prolactin (PRL) in normal resting mammary gland and DMBA-induced mammary adenocarcinoma of female rats was demonstrated with the avidin-biotin-peroxidase complex (ABC) method. Positive staining obtained in the cytoplasm of glandular cells of the mammary gland were more intense on the apical borders of epithelial cells. The immunostaining in the induced tumors was variable, being often less than normal gland cells. The positivity could be seen only in the section fixed in Bouin's solution and treated with the ABC method using a rabbit antiserum against rat PRL. The immunostaining showed a strong positivity in the anterior pituitary tissue of the same animals, and it appeared specific for PRL because of its abolishment when the antiserum absorbed with rat PRL was used. The results reported here emphasize the value of ABC method in the Bouin's solution-fixed sections for detecting hormones in their target tissues.
- 日本組織細胞化学会の論文
著者
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螺良 愛郎
関西医科大学 第2病理
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TSUBURA Airo
Department of Pathology II, Kansai Medical University
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KATO Yuzuru
Department of Internal Medicine, Shimane Medical University
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Oyaizu Naoki
Department Of Pathology Kansai Medical University
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Oyaizu Naoki
関西医科大学 病理
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Oyaizu Naoki
Department Of Clinical Laboratory Medicine Institute Of Medical Science University Of Tokyo
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Oyaizu Naoki
Dept. Of Pathol. Kansai Medical University
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Kato Yasuko
Department Of Anatomy Yamanashi Medical University
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Kato Yuzuru
Department Of Medicine Shimane Medical University
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Tsubura Airo
Kansai Medical Univ. Osaka Jpn
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Tsubura Airo
関西医科大学 病理学第二
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Tsubura Airo
Department Of Pathology 2 Kansai Medical University
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MORII Sotokichi
Department of Pathology, Kansai Medical University
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HARA FUMIO
Department of Pathology, Kansai Medical University
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Kato Y
Department Of Pathology Tokyo Women's Medical University
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螺良 愛郎
Department Of Pathology Ii Kansai Medical University
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Tsubura A
Department Of Pathology Kansai Medical University
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Kato Y
Tokyo Women's Medical Univ.
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Hara F
Sci. Univ. Tokyo Tokyo Jpn
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Hara Fumio
Department Of Pathology Kansai Medical University
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Hara Fumio
Department Of Mechanical Engineering Faculty Of Engineering Science University Of Tokyo
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Morii Sotokichi
Department Of Pathology Kansai Medical School
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Kato Yuzuru
Department Of Anesthesiology Yamagata Prefectural Nihonkai Hospital
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Morii Sotokichi
Department Of Pathology Kansai Medica1 University
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