HRP を取り込んだラット咬筋運動ニューロンのアセチルコリンエステラーゼ合成に関する研究

概要

論文の詳細を見る
Whether or not accumulation of retrogradely transported horseradish peroxidase (HRP) in the neuronal cell bodies affects their metabolic profiles was studied by examining the effects on the synthesis of acetylcholinesterase (AChE) in the rat masseter motoneurons. HRP was injected into the left masseter muscle in the experimental animals, but not injected in the control group. Sixteen hours later all rats were administered diisopropyl fluorophosphate to inactivate the existing AChE and then given an additional 1 hour for the synthesis of new AChE. Serial frozen 60 μm sections of brainstems were processed for AChE activity. The amount of AChE reaction products in the masseter motoneurons was measured by using an image processing system and ratios between the amounts in the right and left masseter motoneurons were calculated. There was no significant difference in the values between the control and experimental groups, suggesting that HRP accumulation in the cell bodies of motoneurons does not affect their synthesis of AChE.
九州歯科学会の論文
1995-12-25